Efficacy of HLA-DR/CD4+ IL-6 and PCT in Predicting Secondary Infection in ICU Patients with Severe Acute Pancreatitis and Guidance for Rational Use of Antimicrobials
GAO Jing, ZHOU Peng, XIE Jing, et al
Weihai Municipal Hospital, Shandong Weihai 264200, China
摘要目的: 分析人类白细胞抗原-DR/CD4+T淋巴细胞(HLA-DR/CD4+)、白细胞介素6(IL-6)及降钙素原(PCT)在ICU重症急性胰腺炎(SAP)继发感染患者中的诊断效能及对抗菌药物合理使用的指导价值。方法: 2020年5月至2023年12月在我院ICU收治的SAP患者80例纳入研究。按照是否继发感染将患者分为感染组(n=43)及未感染组(n=37)。收集所有患者临床资料,包括一般资料、实验室指标、抗菌治疗情况。比较两组一般资料及实验室指标,采用Logistic回归分析重症SAP继发感染的独立危险因素,采用受试者工作特征曲线(ROC)分析血清HLA-DR/CD4+、IL-6及PCT预测SAP继发感染的效能。结果: 感染组APACHE Ⅱ评分大于未感染组(P<0.05)。两组性别、年龄、病因、并发症(高血压、2型糖尿病及高脂血症)情况比较无显著差异(P>0.05)。感染组脂肪酶、IL-6及PCT水平高于未感染组(P<0.05),HLA-DR/CD4+水平低于未感染组(P<0.05)。脂肪酶高表达(OR=2.354,95% CI 1.491~3.716 )、HLA-DR/CD4+高表达(OR=3.508,95% CI 1.283 ~ 9.588)、IL-6高表达(OR=4.284,95% CI 1.469~12.493)及PCT高表达(OR=5.743,95% CI 1.530~21.563)均是SAP继发感染的独立危险因素(P<0.05)。血清HLA-DR/CD4+、IL-6及PCT及三者联合诊断SAP继发感染的AUC值分别为0.809、0.778、0.819及0.959,具有一定预测价值。联合诊断的AUC值及诊断效能明显大于单独HLA-DR/CD4+(z=3.161,P=0.002)、IL-6(z=3.822,P<0.001)及PCT(z=3.346,P=0.001)诊断。局部感染组血清HLA-DR/CD4+水平高于严重感染组,IL-6及PCT水平均低于严重感染组(P<0.05)。局部感染组抗菌药物使用时间、ICU住院时间及总住院时间均较严重感染组显著缩短(P<0.05)。 结论: HLA-DR/CD4+高表达、IL-6高表达及PCT高表达均是SAP继发感染的独立危险因素,可有效预测SAP继发感染。
Abstract：Objective: To investigate the diagnostic efficacy and guidance value for rational use of antimicrobials of human leukocyte antigen-DR/CD4+ T lymphocytes (HLA-DR/CD4+), interleukin-6 (IL-6), and procalcitonin (PCT) in patients with secondary infection of severe acute pancreatitis (SAP) in the ICU. Methods: A total of 80 patients with SAP admitted to the ICU of our hospital from May 2020 to December 2023 were enrolled in the study. Patients were divided into an infection group (n=43) and a non-infection group (n=37) according to whether or not they had secondary infection. Clinical data of all patients were collected, including general information, laboratory indicators, and antimicrobial treatment information. The general information and laboratory indicators of the two groups were compared. Logistic regression analysis was used to analyze the independent risk factors for secondary infection in severe SAP, and receiver operating characteristic (ROC) curve analysis was used to evaluate the efficacy of serum HLA-DR/CD4+, IL-6, and PCT in predicting secondary infection in SAP. Results: The APACHE II score in the infection group was higher than that in the non-infection group (P<0.05). There was no significant difference in gender, age, etiology, and complications (hypertension, type 2 diabetes, and hyperlipidemia) between the two groups (P> 0.05). The levels of lipase, IL-6, and PCT in the infection group were higher than those in the non-infection group (P<0.05), and the level of HLA-DR/CD4+ was lower than that in the non-infection group (P<0.05). Elevated lipase (OR=2.354, 95% CI 1.491~3.716), elevated HLA-DR/CD4+ (OR=3.508, 95% CI 1.283~9.588), elevated IL-6 (OR=4.284, 95% CI 1.469~12.493) and elevated PCT (OR=5.743, 95% CI 1.530~21.563) were all independent risk factors for secondary infection in SAP (P<0.05). The AUC values of serum HLA-DR/CD4+, IL-6, PCT and the three combined for diagnosing SAP secondary infection were 0.809, 0.778, 0.819 and 0.959, respectively, indicating a certain predictive value. The AUC value and diagnostic efficacy of combined diagnosis were significantly higher than those of single HLA-DR/CD4+ (z=3.161, P=0.002), IL-6 (z=3.822, P<0.001) and PCT (z=3.346, P=0.001) diagnosis. The level of serum HLA-DR/CD4+ in the localized infection group was higher than that in the severe infection group, while the levels of IL-6 and PCT were both lower than those in the severe infection group (P<0.05). The duration of antimicrobial use, ICU stay, and total hospital stay in the localized infection group were all significantly shorter than those in the severe infection group (P<0.05). Conclusion: Elevated HLA-DR/CD4+, IL-6, and PCT are all independent risk factors for secondary infection in SAP and can effectively predict secondary infection in SAP.
高婧, 周鹏, 谢晶, 王晓英, 丛铃娟. 人类白细胞抗原-DR/CD4+T淋巴细胞 白细胞介素6联合降钙素原预测ICU重症急性胰腺炎继发感染效能及对抗菌药物合理使用的指导价值[J]. 河北医学, 2024, 30(5): 838-843.
GAO Jing, ZHOU Peng, XIE Jing, et al. Efficacy of HLA-DR/CD4+ IL-6 and PCT in Predicting Secondary Infection in ICU Patients with Severe Acute Pancreatitis and Guidance for Rational Use of Antimicrobials. HeBei Med, 2024, 30(5): 838-843.
[1] Gliem N,Ammer-Herrmenau C,Ellenrieder V,et al.Management of severe acute pancreatitis:an update[J].Digestion,2021,102(4):503-507. [2] Li M,Li N.Clostridium perfringens bloodstream infection secondary to acute pancreatitis:a case report[J].World Clin Cases,2021,9(17):4357-4364. [3] Jin Y,Tao S,Yu G,et al.Predictive value of hyperglycemia on infection in critically ill patients with acute pancreatitis[J].Sci Rep,2023,13(1):4106. [4] Yang X,Zeng J,Yu X,et al.PCT,IL-6,and IL-10 facilitate early diagnosis and pathogen classifications in bloodstream infection[J].Ann Clin Microbiol Antimicrob,2023,22(1):103. [5] Ebik B,Kacmaz H,Tuncel ET,et al.What does the procalcitonin level tell us in patients with acute pancreatitis[J].Coll Physicians Surg Pak,2022,32(10):1272-1277. [6] 中华医学会消化病学分会胰腺疾病学组,《中华胰腺病杂志》编委会,《中华消化杂志》编委会.中国急性胰腺炎诊治指南(2019年,沈阳)[J].临床肝胆病杂志,2019,35(12):2706-2711. [7] Tian H,Chen L,Wu X,et al.Infectious complications in severe acute pancreatitis:pathogens,drug resistance,and status of nosocomial infection in a university-affiliated teaching hospital[J].Dig Dis Sci,2020,65(7):2079-2088. [8] Zhao C,Yao Y,Yao W,et al.Distribution analysis of positive and negative pathogenic bacteria in patients with acute pancreatitis and the clinical characteristics and model prediction analysis of positive infection bacteria[J].Ann Transl Med,2023,11(2):98. [9] Schuetz P,Beishuizen A,Broyles M,et al.Procalcitonin (PCT)-guided antibiotic stewardship:an international experts consensus on optimized clinical use[J].Clin Chem Lab Med,2019,57(9):1308-1318. [10] Minkov G,Dimitrov E,Yovtchev Y,et al.Prognostic value of peripheral blood CD14+HLA-DR/CD4++ monocytes in patients with acute pancreatitis[J].Immunoassay Immunochem,2021,42(5):478-492. [11] 虞竹溪,郝迎迎,刘宁,等.单核细胞HLA-DR/CD4+和T淋巴细胞亚群预测严重创伤继发感染的临床研究[J].中国呼吸与危重监护杂志,2017,16(4):401-403. [12] 李真,赵勇,李俊杰,等.IL-6、PCT、hs-CRP和WBC在新生儿细菌感染性疾病早期诊断中的价值[J].中国现代医学杂志,2022,32(8):1-5.
2024, Vol. 30 
冀公网安备13080202000677号