5-氨基酮戊酸光动力治疗对鲍温病皮损中p53 Caveolin-1表达的影响

doi:10.3969/j.issn.1006-6233.2024.05.016

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摘要 目的: 探讨5-氨基酮戊酸光动力疗法对鲍温病皮损中p53、Caveolin-1表达的影响及意义。方法: 运用5-氨基酮戊酸光动力疗法治疗鲍温病及周围正常皮肤组织各40例,采用免疫组织化学技术(SP法)检测光动力治疗前后鲍温病及周围正常皮肤组织中p53、Caveolin-1的阳性细胞表达率。结果: 治疗前p53蛋白在正常皮肤组织及BD中表达的阳性率分别为10%、40%(χ²=11.202,P<0.001),差异有统计学意义。治疗后p53在正常皮肤组织及BD的阳性率为10%、5%(χ²=0.712,P=0.399),两者间差异无统计学意义。治疗前后p53在BD中的阳性表达率差异有统计学意义(χ²=14.811,P<0.001)。治疗前Caveolin-1在正常皮肤组织及BD中阳性表达率为15%、55%(χ²=14.449,P<0.001),两者间差异有统计学意义。治疗后正常皮肤组织及BD中Caveolin-1的阳性率为5%、12.5%(χ²=0.816,P=0.366),差异无统计学意义。治疗前后Caveolin-1在BD中的阳性表达率差异有统计学意义(χ²=19.013,P<0.001)。BD中p53与Caveolin-1阳性表达呈正相关性(r=0.533,P=0.015)。结论: p53及Caveolin-1的高表达可能与BD的发生密切关联,且ALA-PDT能够抑制p53及Caveolin-1的表达,从而抑制疾病的发展。通过更大样本量的研究,p53及Caveolin-1可能会成为皮肤相关疾病的诊断工具,并为其治疗提供新的靶点。

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关键词 ： 5-氨基酮戊酸光动力疗法, 鲍温病, p53, Caveolin-1

Abstract：Objective: To investigate the influence and significance of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on the expression of p53 and Caveolin-1 in Bowen's disease (BD) lesions. Methods: Forty cases of Bowen's disease and adjacent normal skin tissues were treated with ALA-PDT. Immunohistochemical staining (SP method) was used to detect the positive expression rates of p53 and Caveolin-1 in BD and surrounding normal skin tissues before and after photodynamic therapy. Results: Before treatment, the positive rates of p53 protein expression in normal skin tissue and BD were 10% and 40%, respectively (χ²=11.202, P＜0.001), with a statistically significant difference. After treatment, the positive rates of p53 in normal skin tissue and BD were 10% and 5% (χ²=0.712, P=0.399), respectively, with no statistically significant difference between them. The difference in the positive expression rate of p53 in BD before and after treatment was statistically significant (χ²=14.811, P＜0.001). Before treatment, the positive expression rates of Caveolin-1 in normal skin tissue and BD were 15% and 55%, respectively (χ²=14.449, P＜0.001), with a statistically significant difference. After treatment, the positive rates of Caveolin-1 in normal skin tissue and BD were 5% and 12.5% (χ²=0.816, P=0.366), respectively, with no statistically significant difference between them. The difference in the positive expression rate of Caveolin-1 in BD before and after treatment was statistically significant (χ²=19.013, P＜0.001). There was a positive correlation between p53 and Caveolin-1 expression in BD (r=0.533, P=0.015). Conclusion: The high expression of p53 and Caveolin-1 may be closely related to the occurrence of BD, and ALA-PDT can inhibit the expression of p53 and Caveolin-1, thereby inhibiting the progression of the disease. With a larger sample size, p53 and Caveolin-1 may become diagnostic tools for skin-related diseases and provide new targets for their treatment.

Key words： 5-aminoketovaleric acid photodynamic therapy Bowen's disease p53 Caveolin-1

基金资助:河北省承德市科学技术研究与发展计划项目,(编号:202002A006)

通讯作者: 樊磊强

引用本文:

张艳峰, 樊磊强, 高悦, 孙业晓, 徐冰. 5-氨基酮戊酸光动力治疗对鲍温病皮损中p53 Caveolin-1表达的影响[J]. 河北医学, 2024, 30(5): 794-797.
ZHANG Yanfeng, FAN Leiqiang, GAO Yue, et al. Influence of 5-Aminolevulinic Acid Photodynamic Therapy on the Expression of p53 and Caveolin-1 in Bowen's Disease Lesions. HeBei Med, 2024, 30(5): 794-797.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.05.016 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I5/794

[1] 熊林,陆延娜,万静,等.5-氨基酮戊酸光动力疗法治疗鲍温病10例疗效观察 [J].中国皮肤性病学杂志,2012,26(2):124-125.
[2] O'connell K A,Okhovat J P,Zeitouni N C.Photodynamic therapy for bowen's disease (squamous cell carcinoma in situ) current review and update [J].Photodiagnosis and Photodynamic Therapy,2018,24(12):109-114.
[3] 庄丽珍.氨基酮戊酸光动力疗法治疗皮肤癌前病变及浅表皮肤癌的临床效果及复发率探讨 [J].中华肿瘤防治杂志,2020,27(1):163-165.
[4] 魏云,吴丽娟,,丁高中,等. Caveolin-1、Met蛋白在日光性角化病、鲍温病和皮肤鳞状细胞癌中的表达 [J].中国麻风皮肤病杂志,2019,35(5):276-279.
[5] Morton C,Horn M,Leman J,et al.Comparison of topical methyl aminolevulinate photodynamic therapy with cryotherapy or fluorouracil for treatment of squamous cell carcinoma in situ:results of a multicenter randomized trial [J].Archives of Dermatology,2006,142(6):729-735.
[6] M O,Koskenimies S,Pitknen S.A retrospective study of treatment of squamous cell carcinoma in situ [J].Acta dermato-venereologica,2016,96(1):64-67.
[7] 章申峰,龚兴国.光动力疗法对肿瘤的作用机制及其影响因素 [J].细胞生物学杂志,2005(4):395-399.
[8] Bagazgoitia L,Cuevas J,Juarranza.Expression of p53 and p16 in actinic keratosis,bowenoid actinic keratosis and Bowen's disease [J].Journal of the European Academy of Dermatology & Venereology,2010,24(2):228-230.
[9] Batinac T,Zamolo G,Jonji N,et al.p53 protein expression and cell proliferation in non-neoplastic and neoplastic proliferative skin diseases [J].Tumori,2004,90(1):120.
[10] Talghini S,Halimi M,Baybordi H.Expression of P27,Ki67 and P53 in squamous cell carcinoma,actinic keratosis and bowen disease [J].Pakistan Journal of Biological Sciences Pjbs,2009,12(12):929.
[11] 邹爱玲,石年,石娴,等.p53及p16在皮肤鳞状细胞癌和鲍温病中的表达及意义 [J].中国中西医结合皮肤性病学杂志,2019,18(4):293-298.
[12] Pin F,Fu-chun C,Qi P,et al.The different functions and clinical significances of caveolin-1 in human adenocarcinoma and squamous cell carcinoma [J].Onco Targets Ther,2017(10):819-835.
[13] Cao Wenbo,Zhao,et al.Caveolin-1 affects tumor drug resistance in esophageal squamous cell carcinoma by regulating expressions of P-gp and MRP1 [J].Tumour biology,2016,37(7):9189-9196.
[14] Trimmer C,Sotgia F,Lisanti M P,et al.Cav1 inhibits benign skin tumor development in a two-stage carcinogenesis model by suppressing epidermal proliferation [J].American Journal of Translational Research,2013,5(1):80-91.