Abstract：Objective: To investigate the regulation of berberine on Th cell differentiation and Notch signaling pathway in allergic asthmatic-model mice, as well as the repair of lung tissue injury. Methods: Mice were induced by OVA, and divided into control group, model group, low-dose berberine group, high-dose berberine group, and dexamethasone group after intervention with low and high doses of berberine and dexamethasone. The general conditions of the mice in each group were observed, HE staining was used to observe the inflammatory infiltration of lung tissue, flow cytometry was used to detect the proportions of Th1, Th2, and Th17 cells in lung tissue suspension, ELISA was used to detect the levels of IFN-γ, IL-4, and IL-17 in bronchoalveolar lavage fluid, and Western blot was used to detect the expression of Notch1 and Hes1 protein in lung tissue. Results: Compared with the control group, the mice in the model group had reduced food and water intake, anxiety, mucus in the nose and mouth, obvious inflammation in the lung tissue, decreased Th1 cell proportion and IFN-γ level (P<0.05), increased Th2 and Th17 cell proportions, and IL-4 and IL-17 levels (P<0.05), and increased Notch1 and Hes1 protein expression (P<0.05). Compared with the model group, the mice in the berberine and dexamethasone intervention groups had increased food and water intake, improved mental state, reduced mucus in the nose and mouth, reduced lung tissue inflammation, increased Th1 cell proportion and IFN-γ level (P<0.05), decreased Th2 and Th17 cell proportions, and IL-4 and IL-17 levels (P<0.05), and decreased Notch1 and Hes1 protein expression (P<0.05). Conclusion: Berberine intervention can alleviate lung tissue inflammatory damage in allergic asthma model by regulating Th subset differentiation and inhibiting Notch1 protein expression.
[1] Sim S,Choi Y,Park HS.Immunologic basis of type 2 biologics for severe asthma[J].Immune Netw,2022,22(6):45. [2] 钟新春,朱振刚,刘超武,等.基于STAT1信号通路探讨疏风通络方对支气管哮喘小鼠Th1/Th2失衡的影响[J].中国免疫学杂志,2022,38(22):2729-2734. [3] Zhou M,Qu R,Yin X,et al.Prednisone acetate modulates Th1/Th2 and Th17/Treg cell homeostasis in experimental autoimmune uveitis via orchestrating the Notch signaling pathway[J].Int Immunopharmacol,2023(116):109809. [4] 周晶,董笑克,马青科,等.黄连素对阿尔兹海默症大鼠 Th17,Treg亚群比例及脑组织炎性细胞因子的影响[J].环球中医药,2020,13(8):1304-1308 [5] Su YH,Lin JY.Menthone supplementation protects from allergic inflammation in the lungs of asthmatic mice[J].Eur Pharmacol,2022(931):175222. [6] Luo W,Hu J,Xu W,et al.Distinct spatial and temporal roles for Th1,Th2,and Th17 cells in asthma[J].Front Immunol,2022(13):974066. [7] Nie Y,Yang B,Hu J,et al.Bruceine D ameliorates the balance of Th1/Th2 in a mouse model of ovalbumin-induced allergic asthma via inhibiting the NOTCH pathway[J].Allergol Immunopathol (Madr),2021,49(6):73-79. [8] Piao CH,Fan Y,Nguyen TV,et al.PM2.5 exposure regulates Th1/Th2/Th17 cytokine production through NF-κB signaling in combined allergic rhinitis and asthma syndrome[J].Int Immunopharmacol,2023(119):110254. [9] 付佳,曹宇勃,刘飒.褪黑素调控NF-κB信号通路调节胃癌小鼠Th1/Th2免疫平衡及其机制[J].中国比较医学杂志,2022,32(9):90-96,108. [10] CuiI H,Huang J,Lu M,et al.Antagonistic effect of vitamin E on nAl2O3-induced exacerbation of Th2 and Th17-mediated allergic asthma via oxidative stress[J].Environ Pollut,2019(252):1519-1531. [11] Ghalavand M,Gouvarchin G,Khafaei M,et al.Effect of calcitriol treated mesenchymal stem cells as an immunomodulation micro-environment on allergic asthma in a mouse model[J].Endocr Metab Immune Disord Drug Targets,2023,23(8):1096-1103. [12] Liu L,Wang S,Xing H,et al.Bulleyaconitine a inhibits the lung inflammation and airway remodeling through restoring Th1/Th2 balance in asthmatic model mice[J].Biosci Biotechnol Biochem,2020,84(7):1409-1417. [13] Fu S,Ni S,Wang D,et al.Coptisine suppresses mast cell degranulation and ovalbumin-induced allergic rhinitis[J].Molecules,2018,23(11):3039. [14] Wu JR,He Z,Bao HR,et al.Study on the mechanism of PM2.5 affecting Th1/Th2 immune imbalance through the notch signaling pathway in asthmatic mice[J].Toxicol Res (Camb),2023,12(4):675-684. [15] 王坤,朱慧志,杨磊,等.miR-139下调Notch1/Hes1通路促进骨髓间充质干细胞归巢于哮喘大鼠肺组织抑制Th2细胞炎症反应[J].细胞与分子免疫学杂志,2021,37(2):97-104.
2024, Vol. 30 
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