Abstract：Objective: To investigate the relationship between the expression of serum miR-33 and ABCA1 in patients with gestational diabetes and the condition and pregnancy outcome. Methods: Retrospective analysis was made on 75 pregnant women (diabetes group) who were diagnosed with gestational diabetes during obstetric examination in our hospital from April 2022 to April 2023, and 64 pregnant women with normal pregnancy as the control group, fasting blood samples were taken and insulin resistance was calculated; reverse transcription quantitative real-time PCR method was applied to measure the expression level of serum miR-33; enzyme linked immunosorbent assay (ELISA) was applied to measure serum ABCA1 level; the serum levels of miR-33 and ABCA1 were compared between two groups and among patients with different Priscilla WHITE grades; Pearson method was applied to analyze the correlation between serum miR-33 and ABCA1 levels, and their correlation with insulin resistance; the adverse outcomes of subjects and newborns in two groups were compared; according to the adverse pregnancy outcome, the patients with gestational diabetes were separated into good pregnancy group (42 cases) and poor pregnancy group (33 cases), the serum levels of ABCA1 and miR-33 were compared between the two groups; Logistic regression was applied to analyze the factors affecting the outcome of gestational diabetes. Results: The serum ABCA1 level in the diabetes group was obviously lower than that in the control group (t=9.839, P=0.000), and the expression of miR-33 and insulin resistance were obviously increased (t=9.256, P=0.000), there was no statistical difference in the gestational week, first pregnancy, body mass and age (P>0.05), as the condition worsened, the expression level of miR-33 increased (t=5.231, P=0.000) and the level of ABCA1 decreased (t=9.421, P=0.000). Serum ABCA1 was negatively correlated with miR-33 and insulin resistance in patients with gestational diabetes (r=-0.685, -0.510, P=0.000), but miR-33 was positively correlated with insulin resistance (r=0.535, P=0.000); the incidences of macrosomia, neonatal hypoglycemia and premature rupture of membranes in the diabetes group were obviously higher than those in the control group (P<0.05); the serum miR-33 level in the poor pregnancy group were obviously increased compared to the good pregnancy group (P<0.05), but the ABCA1 level was obviously reduced (P<0.05); serum miR-33 and ABCA1 levels and insulin resistance were independent influencing factors for adverse pregnancy in patients with gestational diabetes (P<0.05). Conclusion: Serum miR-33 is obviously increased and ABCA1 expression is decreased in patients with gestational diabetes, both of which are related to insulin resistance. The pregnancy outcome is affected by the levels of serum miR-33, ABCA1 and insulin resistance.
李红霞, 曾欢. 妊娠期糖尿病患者血清miR-33 ABCA1表达与病情及妊娠结局的关系[J]. 河北医学, 2024, 30(4): 570-575.
LI Hongxia, ZENG Huan. Relationship between the Expression of Serum miR-33 ABCA1 in Patients with Gestational Diabetes and the Condition and Pregnancy Outcome. HeBei Med, 2024, 30(4): 570-575.
2024, Vol. 30 
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