Impacts of Polysaccharide of Trametes Robiniophila Murr on the Malignant Biological Behavior of Cervical Cancer Cells by Regulating the SPHK1/S1P/S1PR3 Signaling Pathway
LI Lipin, MA Suyan, AN Ruzheng
Shijiazhuang Ping'an Hospital, Hebei Shijiazhuang 050000, China
Abstract：Objective: To investigate the impacts of Huaier polysaccharide (HP) regulating the SPHK1/S1P/S1PR3 signaling pathway on the malignant biological behavior of cervical cancer cells.Methods: MTT was used to detect the viability of cervical cancer cells treated with Sophora auricula polysaccharides (0, 25, 50, 100, 200, 400μg/mL) and screen for the optimal drug concentration. The subjects were divided into control group, low, medium, and high concentration groups of Huaier polysaccharide (HP-L, HP-M, HP-H groups), and high concentration of Huaier polysaccharide+SphK1 activator K6PC-5 group (HP-H+K6PC-5 group). The cell proliferation, migration and invasion were observed; Western blot was used to detect the protein levels of SPHK1, S1P, S1PR3, Snail, N-cadherin, and E-cadherin. Results: After treatment for 24, 48 and 72h, HP treatments of 50μg/mL, 100μg/mL, 200μg/mL and 400μg/mL significantly decreased cell viability compared with 0μg/mL (P<0.05), Edu positive rate, number of invasive cells, scratch healing rate and Snail, N-cadherin, SPHK1, S1P and S1PR3 levels in HP-L, HP-M and HP-H groups were obviously lower than those in Control group (P<0.05), E-cadherin levels significantly increase (P<0.05). The Edu positive rate, number of invasive cells, scratch healing rate and Snail, N-cadherin, SPHK1, S1P and S1PR3 levels in HP-H+K6PC-5 group were obviously higher than those in HP-H group (P<0.05), E-cadherin levels significantly decrease (P<0.05). Conclusion: HP may inhibit the proliferation, invasion and migration of cervical cancer cells by inhibiting SPHK1/S1P/S1PR3 signaling pathway.
李丽品, 马素艳, 安入征. 槐耳多糖调节SPHK1/S1P/S1PR3信号通路对宫颈癌细胞恶性生物学行为的影响[J]. 河北医学, 2024, 30(3): 411-415.
LI Lipin, MA Suyan, AN Ruzheng. Impacts of Polysaccharide of Trametes Robiniophila Murr on the Malignant Biological Behavior of Cervical Cancer Cells by Regulating the SPHK1/S1P/S1PR3 Signaling Pathway. HeBei Med, 2024, 30(3): 411-415.
[1] Fan H,He YJ,Xiang JQ,et al.ROS generation attenuates the anti-cancer effect of CPX on cervical cancer cells by inducing autophagy and inhibiting glycophagy[J].Redox Biol,2022,53(1):102339-102349. [2] Qi J,Xie FJ,Liu S,et al.Huaier granule combined with tegafur gimeracil oteracil potassium promotes stage Ⅱb gastric cancer prognosis and induces gastric cancer cell apoptosis by regulating livin[J].Biomed Res Int,2020,2020(1):1-10. [3] Li F,Zhang YF,Lin ZJ,et al.Targeting SPHK1/S1PR3-regulated S-1-P metabolic disorder triggers autophagic cell death in pulmonary lymphangiomyomatosis (LAM)[J].Cell Death Dis,2022,13(12):1065-1075. [4] Di Paolo A,Vignini A,Alia S,et al.Pathogenic role of the sphingosine 1-phosphate (S1P) pathway in common gynecologic disorders (GDs):a possible novel therapeutic target[J].Int Mol Sci,2022,23(21):13538-13548. [5] Sukocheva OA,Furuya H,Ng ML,et al.Sphingosine kinase and sphingosine-1-phosphate receptor signaling pathway in inflammatory gastrointestinal disease and cancers:a novel therapeutic target[J].Pharm Ther,2020,207(1):107464-107550. [6] Shoaib S,Islam N,Yusuf N.Phytocompounds from the medicinal and dietary plants:multi-target agents for cervical cancer prevention and therapy[J].Curr Med Chem,2022,29(26):4481-4506. [7] Hu BQ,Yan WT,Wang MH,et al.Huaier polysaccharide inhibits the stem-like characteristics of ERα-36high triple negative breast cancer cells via inactivation of the ERα-36 signaling pathway[J].Int Biol Sci,2019,15(7):1358-1367. [8] 张泽敏,庞岚.槐耳多糖抑制AEG-1表达对子宫内膜癌细胞生物学行为的影响[J].川北医学院学报,2023,38(10):1299-1305. [9] Huang XT,Xiang LY,Wang BY,et al.CMTM6 promotes migration invasion and EMT by interacting with and stabilizing vimentin in hepatocellular carcinoma cells[J].Transl Med,2021,19(1):120-130. [10] Fang L,Zhang YZ,Zang YW,et al.HP-1 inhibits the progression of ccRCC and enhances sunitinib therapeutic effects by suppressing EMT[J].Carbohydrate Polymers,2019,223(1):115109-115121. [11] 雷蕾,高彦茹,蔡洲,等.槐耳多糖通过抑制AKT/GSK3β/Snail信号通路抑制胰腺癌细胞增殖和上皮间质转化[J].现代肿瘤医学,2023,31(24):4536-4541. [12] Khoei SG,Sadeghi H,Samadi P,et al.Relationship between Sphk1/S1P and microRNAs in human cancers[J].Biotechnol Appl Biochem,2021,68(2):279-287. [13] Zheng XJ,Li W,Ren LW,et al.The sphingosine kinase-1/sphingosine-1-phosphate axis in cancer:Potential target for anticancer therapy[J].Pharmacol Ther,2019,195(1):85-99. [14] Zhang Y,Cheng L,Shi X,et al.The sphingosine kinase inhibitor SKI-V suppresses cervical cancer cell growth[J].Int Biol Sci,2022,18(7):2994-3005.
2024, Vol. 30 
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