Abstract：Objective: To explore the impact of miR-145-5p targeting Survivin and the apoptotic signaling pathway on apoptosis, invasion, and migration in esophageal cancer.Methods: Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to detect the relative expression levels of miR-145-5p and Survivin mRNA in esophageal cancer tissues and cell lines. A dual-luciferase reporter gene analysis was conducted to validate the targeted regulatory relationship between miR-145-5p and Survivin. RT-qPCR was used to measure the relative expression levels of miR-145-5p and Survivin mRNA after cell transfection. Western blot was performed to assess the expression of BCL2, MDM2, and Caspase-3 proteins in the apoptotic pathway. Cell survival, apoptosis rate, migration rate, and invasion ability were evaluated using MTT, flow cytometry, scratch healing, and Transwell chamber experiments in TE-1 cells. Results: Compared to adjacent normal tissues, miR-145-5p was downregulated, while Survivin was upregulated in cancer tissues (P<0.05). In comparison to normal esophageal mucosal epithelial HEEC cells, TE-1 cells exhibited low expression of miR-145-5p and high expression of Survivin (P<0.05). Dual-luciferase reporter gene experiments confirmed the targeted regulatory relationship between miR-145-5p and Survivin. Transfection with miR-145-5p mimic significantly increased miR-145-5p expression, inhibited Survivin mRNA expression, downregulated BCL2 and MDM2 protein expression, upregulated Caspase-3 expression, promoted cell apoptosis, and inhibited cell viability, invasion, and migration compared to untransfected and miR-145-5p control-transfected TE-1 cells (P<0.05). Conclusion: Through negative regulation by miR-145-5p, the expression of Survivin is inhibited, leading to the activation of the apoptotic pathway. This promotes cell apoptosis and inhibits the survival, invasion, and migration capabilities of esophageal cancer cells.
郑竞雄, 杨阳, 孙光蕊, 赵宝山, 侯继申, 梁宗英, 王敏. miR-145-5p调控Survivin通过凋亡通路对食管癌生物学行为的影响[J]. 河北医学, 2024, 30(3): 386-392.
ZHENG Jingxiong, et al. Impact of miR-145-5p-Mediated Regulation of Survivin via the Apoptotic Pathway on the Biological Behavior of Esophageal Cancer. HeBei Med, 2024, 30(3): 386-392.
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2024, Vol. 30 
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