Abstract：Objective: To investigate the effect of pinocembrin (Pin) on the malignant progression of esophageal squamous carcinoma cells and its regulatory mechanism on the Janus activated kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway.Methods: Human esophageal squamous carcinoma cell line KYSE-410 was randomly separated into KYSE-410 group, Pin-low concentration (Pin-L) group, Pin-medium concentration (Pin-M) group, Pin-high concentration (Pin-H) group, and Pin-H+JAK2 activator (Pin-H+Broussonin E) group. CCK-8 method and clone plate assay were applied to detect the proliferative activity of cells in each group. Transwell experiment was applied to detect the migration and invasion abilities of cells; flow cytometry was applied to detect the apoptosis of cells in each group; Western blot was applied to detect the expression levels of JAK2/STAT3 signaling pathway related proteins of cells in each group. Results: Compared with the KYSE-410 group, the cell survival rates (79.91±2.31, 62.33±1.41, 51.17±1.05, F=307.400), the number of clone cells formed (162.82±6.33, 144.59± 5.09, 120.18±3.72, F=209.800), and the number of migrated cells (77.73±3.26, 60.83±2.41, 49.28±1.60, F=360.314) were observed in the Pin-L, Pin-M, and Pin-H groups. The number of invasive cells (62.72±2.42, 50.93±2.07, 32.47±1.55, F=460.221), as well as p-JAK2/JAK2 (0.77±0.06, 0.60±0.04, 0.42±0.03, F=68.226) and p-STAT3/STAT3 values (0.70±0.06, 0.58±0.04, 0.39±0.03, F=61.160) in the cells, decreased (P<0.001), while the apoptosis rate (24.72±2.18, 39.62±3.66, 48.33±4.13, F=235.11) increased (P<0.001), the addition of Broussonin E reversed the inhibitory effect of Pin on the malignant progression of KYSE-410 cells (P<0.05). Conclusion: Pin can inhibit the malignant progression of esophageal squamous carcinoma cells, and its mechanism of action may be related to the inhibition of the JAK2/STAT3 signaling pathway.
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2024, Vol. 30 
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