Abstract:Objective: To establish a guinea pig cholelithiasis model using biliary bacteria from patients with recurrent common bile duct stones, providing a clinically relevant animal model for investigating the role of biliary microbiota in the recurrence of common bile duct stones and the formation of cholelithiasis. Methods: Thirty-five Dunkin Hartley guinea pigs, regardless of gender, were used in two stages of experiments (20 in the first stage and 15 in the second stage). Biliary bacteria from patients with recurrent common bile duct stones were introduced by biliary catheterization. In the first stage, observations were made at the 9th week post-modeling, including postoperative survival rate, bile characteristics, and intrahepatic and extrahepatic stone formation in the animal model. Bile samples were collected for bacterial culture. In the second stage, two animals were sacrificed daily from the 2nd day post-modeling to observe bile characteristics and stone formation in the biliary system. Pathological changes in the liver, gallbladder, and common bile duct tissues of gallstone-bearing animals were examined, and the feasibility of the modeling method for establishing a cholelithiasis animal model was comprehensively evaluated. Results: Compared to previous modeling methods using biliary catheterization with injection of Escherichia coli combined with incomplete obstruction, the method used in this study was more consistent with the biliary microbial status of patients with recurrent common bile duct stones during stone recurrence and cholelithiasis formation. Moreover, it had a shorter modeling period, higher model success rate, relatively lower operational difficulty, and lower animal mortality rate. Conclusion: The modeling method used in this study successfully established a guinea pig cholelithiasis model, providing a reliable animal model for studying the mechanisms underlying the role of biliary microbiota in the recurrence of common bile duct stones and the formation of cholelithiasis after ERCP procedures.
卞昊宇, 韩冉, 段学光, 姚玉璞, 吕福琪, 魏骄阳, 张立平. 运用复发型胆总管结石患者胆道细菌建立豚鼠胆石症模型[J]. 河北医学, 2024, 30(1): 40-45.
BIAN Haoyu, HAN Ran, DUAN Xueguang, et al. Establishment of a Guinea Pig Cholelithiasis Model Using Biliary Bacteria from Recurrent Common Bile Duct Stone Patients. HeBei Med, 2024, 30(1): 40-45.
[1] Li S,Su B,Chen P,et al.Risk factors for recurrence of common bile duct stones after endoscopic biliary sphincterotomy[J].Int Med Res,2018,46(7):2595-2605. [2] 安爱虎,周文策,张辉.胆道微生态与胆总管结石形成关系的研究进展[J].肝胆胰外科杂志,2019,31(8):509-513. [3] 杨河,符敏,胡军,等.胆总管结石患者胆汁和肠道病原菌特点及其与结石复发的相关性[J].中国普通外科杂志,2022,31(3):416-420. [4] T M.Pathogenesis of calcium bilirubinate gallstone:Role of E.Coli,beta-glucuronidase and coagulation by inorganic ions,polyelectrolytes and agitation[J].Ann Surg,1966,164(1):90-100. [5] 杜国涛,王青,赵伟伟,等.胆、肠菌群差异性与胆囊胆固醇结石形成关系[J].肝胆胰外科杂志,2022,34(3):138-144. [6] 谭文惠,陈睿妍,吴嘉钏,等.复发型胆总管结石患者胆道微生物的特征[J].新医学,2021,52(7):513-517. [7] 武金都.胆管结石患者胆道微生态与oddis括约肌相关性研究[D]:青海大学,2022. [8] 廖原.胆石症患者胆汁的胆汁酸代谢及胆道菌群变化研究[D]:郑州大学,2021. [9] 文乐,钟国跃,李星,等.胆石症实验动物模型及其中药治疗研究进展[J].中成药,2018,40(9):2040-2044. [10] Tsai WL,Lai KH,Lin CK,et al.Composition of common bile duct stones in Chinese patients during and after endoscopic sphincterotomy[J].World Gastroenterol,2005,11(27):4246-4249. [11] Kim KH,Rhu JH,Kim TN.Recurrence of bile duct stones after endoscopic papillary large balloon dilation combined with limited sphincterotomy:long-term follow-up study[J].Gut Liver,2012,6(1):107-112.