Abstract：Objective: To explore the therapeutic effect of total saponins of American ginseng on stress urinary incontinence by regulating Nrf2/ARE pathway. Methods: A stress urinary incontinence rat model was established.Sixty rats were randomly assigned to the following groups:Control group,Stress Urinary Incontinence (SUI) group,and SUI groups treated with low,medium,and high doses of American Ginseng Total Saponins (PQSL,PQSM,PQSH).Additionally,one SUI group received Nrf2-specific inhibitor ML385 (PQSH+ML385).Each group consisted of 10 rats.The positive sneezing rates were recorded.Urodynamic parameters,including MBC,LPP,ALPP,as well as intravesical pressure (voided volume,residual volume,voiding efficiency) were measured.Masson staining was used to assess collagen fiber levels in urethral tissues.Oxidative stress indicators (MDA,SOD,GSH-Px) were determined in urethral tissues.Western blot analysis was performed to assess the expression of Nrf2,HO-1,and NQO1 in brain tissue. Results: In the Control group,sneezing tests were consistently negative.In the SUI group,all 50 rats showed positive sneezing test results.The positive sneezing rates in PQSL,PQSM,and PQSH groups were 60% (6/10),40% (4/10),and 20% (2/10),respectively,whereas in the PQSH+ML385 group,the rate was 70% (7/10),and in the SUI group,it was 80% (8/10).Compared to the Control group,the SUI group exhibited reduced MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,along with increased urine volume,residual volume,and MDA content (P<0.05).In comparison with the SUI group,the PQSL,PQSM,and PQSH groups demonstrated elevated MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,and reduced urine volume,residual volume,and MDA content,with effects showing concentration-dependent patterns (P<0.05).When compared to the PQSH group,the PQSH+ML385 group displayed decreased MBC,LPP,ALPP levels,urinary efficiency,GSH-Px content,and SOD activity,alongside increased urine volume,residual volume,and MDA content (P<0.05).Compared to the Control group,the SUI group showed significantly decreased expression of Nrf2,HO-1,and NQO1 in urethral tissue (P<0.05).In contrast,the PQSL,PQSM,and PQSH groups exhibited significantly increased expression of Nrf2,HO-1,and NQO1 in urethral tissue,with effects being dose-dependent (P<0.05).When compared to the PQSH group,the PQSH+ML385 group showed significantly decreased expression of Nrf2,HO-1,and NQO1 in urethral tissue (P<0.05). Conclusion: American Ginseng Total Saponins may improve urinary function in stress urinary incontinence by reducing oxidative stress in the urethra.This effect appears to be associated with the activation of the Nrf2-ARE signaling pathway.
杨丹丹, 唐红英, 张蝶, 魏国斌, 张靖雪, 李南希. 西洋参总皂苷通过Nrf2-ARE通路对压力性尿失禁的治疗作用[J]. 河北医学, 2023, 29(11): 1805-1811.
YANG Dandan, TANG Hongying, ZHANG Die, et al. Therapeutic Effects of American Ginseng Total Saponins on Stress Urinary Incontinence via Modulation of the Nrf2-ARE Pathway. HeBei Med, 2023, 29(11): 1805-1811.
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