Abstract：Objective: To identify miRNAs regulated by Helicobacter pylori infection and their mechanism of action in gastric cancer. Methods: Expression levels of miR-125a-5p and EVA1A were assessed using qRT-PCR in Hp-infected patient tissues.The biological functions of the miR-125a-5p/EVA1A axis in gastric cancer cell proliferation,migration and invasion were investigated using cell functional experiments after Hp infection.The binding relationship between miR-125a-5p and EVA1A was confirmed through a dual luciferase assay. Results: miR-125a-5p was down-regulated in Hp-infected patient tissues and Hp-infected gastric cancer cells.Functional experiments revealed that the overexpression of miR-125a-5p could inhibit gastric cancer cell proliferation and invasion.The targeting of miR-125a-5p down-regulated the expression of EVA1A,and overexpressing EVA1A could reverse the effects of miR-125a-5p overexpression on gastric cancer phenotype. Conclusion: Hp-induced miR-125a-5p,which targets EVA1A,inhibits the proliferation and invasion of gastric cancer.MiR-125a-5p and EVA1A may represent potential therapeutic targets for gastric cancer.
[1] Chen D,Liu Z,Liu W,et al.Predicting postoperative peritoneal metastasis in gastric cancer with serosal invasion using a collagen nomogram[J].Nat Commun,2021,12(1):179. [2] Najah H,Malgras B,Dohan A,et al.The role of single-incision laparoscopic peritoneal exploration in the management of patients with peritoneal metastases[J].Surg Endosc,2020,34(5):2040-2049. [3] Smyth EC,Nilsson M,Grabsch HI,et al.Gastric cancer[J].Lancet,2020,396(10251):635-648. [4] Washington K.7th edition of the AJCC cancer staging manual:stomach[J].Ann Surg Oncol,2010,17(12):3077-3079. [5] Sung H,Ferlay J,Siegel RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J].CA Cancer Clin,2021,71(3):209-249. [6] Yang Z,Guo X,Li G,et al.Long noncoding RNAs as potential biomarkers in gastric cancer:opportunities and challenges[J].Cancer Lett,2016,371(1):62-70. [7] Ishaq S,Nunn L.Helicobacter pylori and gastric cancer:a state of the art review[J].Gastroenterol Hepatol Bed Bench,2015,8(Suppl 1):6-14. [8] Karimi P,Islami F,Anandasabapathy S,et al.Gastric cancer:descriptive epidemiology,risk factors,screening,and prevention[J].Cancer Epidemiol Biomarkers Prev,2014,23(5):700-713. [9] Liu L,He J,Wei X,et al.MicroRNA-20a-mediated loss of autophagy contributes to breast tumorigenesis by promoting genomic damage and instability[J].Oncogene,2017,36(42):5874-5884. [10] He B,Zhao Z,Cai Q,et al.miRNA-based biomarkers,therapies,and resistance in cancer[J].Int Biol Sci,2020,16(14):2628-247. [11] Liu H,Xiang Y,Zong QB,et al.miR-6745-TIMP1 axis inhibits cell growth and metastasis in gastric cancer[J].Aging (Albany NY),2021,13(21):24402-24416. [12] Deng P,Li K,Gu F,et al.LINC00242/miR-1-3p/G6PD axis regulates warburg effect and affects gastric cancer proliferation and apoptosis[J].Mol Med,2021,27(1):9.
2023, Vol. 29 
冀公网安备13080202000786号