Abstract：Objective: To discuss the clinical significance of microRNA-29b-3p (miR-29b-3p) and forkhead box O3A (FOXO3A) in colon cancer tissues by detecting their expression level. Methods: A total of 82 patients with colon cancer treated in our hospital from January 2015 to January 2017 were selected. Colon cancer tissues and adjacent normal tissues ≥5cm from the tumor margin were collected during the operation. Real-time fluorescence quantification (qRT-PCR) was used to detect the expression levels of miR-29b-3p and FOXO3A mRNA in various tissues, at the same time, Western blot was used to detect the expression level of FOXO3A protein in each tissue. Pearson method was used to analyze the correlation between miR-29b-3p and FOXO3A mRNA expression in colon cancer; Kaplan-Meier method was used to analyze the relationship between the expression of miR-29b-3p, FOXO3A mRNA and the survival rate of patients; the factors affecting the 5-year mortality of patients with colon cancer were analyzed by COX regression. Results: The expression level of miR-29b-3p in colon cancer tissues (1.55±0.40) was higher than that in adjacent normal tissues (1.01±0.23). The expression levels of FOXO3A mRNA and protein in colon cancer tissues [(0.48±0.13), (0.72±0.19)] were lower than those in adjacent normal tissues [(0.99±0.25), (1.43±0.37)]. The difference was statistically significant (t=23.87, 35.501, 33.318, P<0.05). The expressions of miR-29b-3p and FOXO3A mRNA in colon cancer were correlated with TNM stage and lymph node metastasis of colon cancer patient (P<0.05). There was a negative correlation between the expression of miR-29b-3p and FOXO3A mRNA in colon cancer (r=-0.479, P<0.05). The 5-year overall survival rate of patients with low expression of miR-29b-3p (83.33%) was higher than that of patients with high expression (55.00%), and the difference was statistically significant (χ2=6.820, P<0.05). The 5-year overall survival rate of patients in the FOXO3A mRNA high-expression group (88.64%) was higher than that of patients in the low-expression group (47.37%), and the difference was statistically significant (χ2=16.005, P<0.05). The high expression of miR-29b-3p and low expression of FOXO3A mRNA in TNM Ⅲ stage and colon cancer tissues were risk factors for 5-year death in colon cancer patients (P<0.05). Conclusion: The expression of miR-29b-3p is high, and the expression of FOXO3A mRNA and its protein is low in colon cancer tissues. miR-29b-3p and FOXO3A mRNA are correlated with TNM stage, lymph node metastasis, and prognosis.
李博文, 韩元圣, 黄高俊, 郑磊. miR-29b-3p和FOXO3A在结肠癌组织中的表达及临床意义[J]. 河北医学, 2023, 29(10): 1623-1629.
LI Bowen, HAN Yuansheng, HUANG Gaojun, et al. Expression and Clinical Significance of miR-29b-3p and FOXO3A in Colon Cancer Tissues. HeBei Med, 2023, 29(10): 1623-1629.
[1] Peng Z,Chen J,Cao W,et al.The expression and clinical significance of TROP-2 protein in colon cancer[J].Medicine,2020,99(3):18791. [2] Alisyeda Z,Langden S S S,Munkhzul C,et al.Regulatory mechanism of microRNA expression in cancer[J].Int Mol Sci,2020,21(5):1723. [3] He J,Ye W,Kou N,et al.MicroRNA-29b-3p suppresses oral squamous cell carcinoma cell migration and invasion via IL32/AKT signalling pathway[J].Cell Mol Med,2020,24(1):841-849. [4] 周静,王静萱.转录因子叉头框O促进肿瘤发生、发展的作用及机制研究进展[J].癌症进展,2020,18(4):343-346,428. [5] Penrose H M,Cable C,Heller S,et al.Loss of forkhead box O3 facilitates inflammatory colon cancer:transcriptome profiling of the immune landscape and novel targets[J].Cell Mol Gastroenterol Hepatol,2019,7(2):391-408. [6] Yang Q,Yu W,Han X.Overexpression of microRNA101 causes antitumor effects by targeting CREB1 in colon cancer[J].Mol Med Rep,2019,19(4):3159-3167. [7] 唐金舟,毛爱红,廖世奇.miRNA-29b在癌症发生发展中的作用机制及其临床应用的研究进展[J].中国肿瘤生物治疗杂志,2019,147(12):119-125. [8] Li C,Wang P,Du J,et al.LncRNA RAD51-AS1/miR-29b/c-3p/NDRG2 crosstalk repressed proliferation,invasion and glycolysis of colorectal cancer[J].IUBMB Life,2021,73(1):286-298. [9] 陈发帅,薛长年,刘世佳,等.E-cadherin和FOXO3a在胃癌组织和细胞中表达的关系[J].中国病理生理杂志,2019,35(3):66-71. [10] 张翠萍,邹佳芮,解水杉,等.IKBKE和FOXO3在结直肠癌中的表达及临床意义分析[J].重庆医学,2020,49(7):1110-1113,1117. [11] Song Y,Zeng S,Zheng G,et al.FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis[J].Oncogene,2021,40(4):777-790.
2023, Vol. 29 
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