Abstract：Objective: To investigate the effect of esculetin (ESC) on the oxidative stress induced by doxorubicin(DOX) in cardiomyocytes. Methods: SD rats were divided into control group, DOX group, ESC low dose and high dose + DOX group. The serum myocardial enzymes and SOD, MDA levels in myocardial tissue were detected in each group. The changes of collagen fibers in myocardial tissue were compared by Masson staining. The expression of related proteins in each group was detected by Western blot. Results: ESC can increase the SOD level and decrease the increase of myocardial enzyme and MDA caused by DOX (P<0.05); after DOX intervention, the collagen fiber in myocardial tissue increased significantly, while the collagen fiber in myocardial tissue pre-treated by ESC decreased significantly; ESC can reduce the increase of p53 content caused by DOX (P<0.05), and up-regulate the antioxidant enzyme HO-1 (P<0.05), MDM2 (P<0.05) and Nrf2(P<0.05). Conclusion: Ganoderma polysaccharide can improve the oxidative stress injury of myocardial tissue induced by doxorubicin and play a protective role in myocardial tissue.
2023, Vol. 29 
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