Abstract：Objective: To investigate the expression of hepatocyte growth factor -1 (PTP4A1) in glioma and effect on the apoptosis of glioma cells. Methods: Tissue protein in brain glioma and tumor adjacent tissues were extracted by total protein extraction kit; PTP4A1 protein expression were detected by western blot; siRNA-NC and PTP4A1-siRNA was transfected into the human glioma U251 cells of the logarithmic growth phase, and without any siRNA transfection as the control group, each group of cells transfected for 48h, expression of PTP4A1, Ki67, PCNA, Cleaved, Caspase3, β-catenin, CyclinD1, C-myc protein were detected by Western blot; cell proliferation was detected by CCK8 assay; cell apoptosis was detected by flow cytometry. Results: The expression of PTP4A1 protein in gliomas was significantly higher than that in the tumor adjacent tissues (P<0.01); the expression of PTP4A1 protein in siRNA-NC group and control group had no significant difference (P>0.05), expression of PTP4A1 protein in PTP4A1-siRNA group were significantly lower than the control group (P<0.01); compared with the control group and siRNA-NC group, the cell survival rate and the expression of Ki67, PCNA, β-catenin, CyclinD1, C-myc protein in PTP4A1-siRNA group were significantly decreased, cell apoptosis rate and expression of Cleaved Caspase3 protein was significantly increased (P<0.01). Conclusion: PTP4A1 is higher expression in glioma tissues. And silented PTP4A1 expression can inhibit the proliferation and induce apoptosis of human glioma U251 cells by down regulating the Wnt/β -catenin signaling pathway.
权俊杰, 屈建强, 周乐. 肝细胞再生磷酸因子-1在脑胶质瘤组织中的表达及对脑胶质瘤细胞凋亡的影响[J]. 河北医学, 2017, 23(5): 724-727.
QUAN Junjie, QU Jianqiang, ZHOU Le, et al. Expression of Hepatocyte Growth Factor-1 in Human Glioma and Effect on Apoptosis of Glioma Cells. 河北医学, 2017, 23(5): 724-727.
[1] Choi C, Raisanen J M, Ganji S K, et al. Prospective longitudinal analysis of 2-hydroxyglutarate magnetic resonance spectroscopy identifies broad clinical utility for the management of patients with IDH-mutant glioma[J].Journal of Clinical Oncology, 2016, 34(33): 4030~4039. [2] Ceccarelli M, Barthel F P, Malta T M, et al. Molecular profiling reveals biologically discrete subsets and pathways of progression in diffuse glioma[J].Cell, 2016, 164(3): 550~563. [3] Lu Z, Guo H, Lin Y, et al. Effects of PTEN gene silencing on invasion and EMT in oral squamous carcinoma Tca8113 cells[J].Journal of Oral Pathology & Medicine, 2017, 46(1): 31~38. [4] Rubio T. Regulatory mechanisms of phosphatase of regenerating liver (PRL)-3[J].Biochemical Society Transactions, 2016, 44(5): 1305~1312. [5] Huang X, Jiao S F, Zhu F C, et al. Construction of a RNAi lentiviral vector targeting ARK5 gene and its effect on the biological behavior of gastric cancer SGC7901 cells[J].Zhonghua zhong liu za zhi, 2016, 38(2): 93~99. [6] Yan Y, Liu L, Xiong H, et al. Functional polyesters enable selective siRNA delivery to lung cancer over matched normal cells[J].Proceedings of the National Academy of Sciences, 2016, 113(39): E5702~E5710. [7] Flores-Pérez A, Marchat L A, Rodríguez-Cuevas S, et al. Suppression of cell migration is promoted by miR-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells[J].BMC Cancer, 2016, 16(1): 379. [8] Lee J D, Jung H, Min S H. Identification of proteins suppressing the functions of oncogenic phosphatase of regenerating liver 1 and 3[J].Experimental and Therapeutic Medicine, 2016, 12(5): 2974~2982. [9] Nakayama N, Yamashita K, Tanaka T, et al. Genomic gain of the PRL-3 gene may represent poor prognosis of primary colorectal cancer, and associate with liver metastasis[J].Clinical & Experimental Metastasis, 2016, 33(1): 3~13. [10] Horiguchi K, Nakakura T, Yoshida S, et al. Identification of THY1 as a novel thyrotrope marker and THY1 antibody-mediated thyrotrope isolation in the rat anterior pituitary gland[J].Biochemical and Biophysical Research Communications, 2016, 480(2): 273~279. [11] Voena C, Varesio L M, Zhang L, et al. Oncogenic ALK regulates EMT in non-small cell lung carcinoma through repression of the epithelial splicing regulatory protein 1[J].Oncotarget, 2016, 7(22): 33316. [12] Gungabeesoon J, Tremblay M L, Uetani N. Localizing PRL-2 expression and determining the effects of dietary Mg2+[J].Histochemistry and Cell Biology, 2016, 146(1): 99~111. [13] Kopsiaftis S, Sullivan K L, Garg I, et al. AMPK 2 Regulates Bladder Cancer Growth through SKP2-Mediated Degradation of p27[J].Molecular Cancer Research, 2016, 14(12): 1182~1194. [14] Abubakar M, Howat W J, Daley F, et al. High‐throughput automated scoring of Ki67 in breast cancer tissue microarrays from the Breast Cancer Association Consortium[J].The Journal of Pathology: Clinical Research, 2016, 2(3): 138~153. [15] Nguyen P H, Giraud J, Staedel C, et al. All-trans retinoic acid targets gastric cancer stem cells and inhibits patient-derived gastric carcinoma tumor growth[J].Oncogene, 2016, 35(43): 5619~5628. [16] Gu L, Hickey R J, Reckamp K L, et al. Structural analysis identifies an orally active PCNA inhibitor that inhibits the growth of small cell lung cancer cells without causing significant toxicity to nonmalignant cells[J].Journal of Thoracic Oncology, 2016, 11(2): S22~S23. [17] 申建刚, 张定国, 朱惠明. 血管内皮生长因子-A siRNA 对人肝癌 HepG2 细胞凋亡的影响及机制[J].中国老年学杂志, 2016, 36(03): 542~544. [18] Liu C, Vojnovic D, Kochevar I E, et al. UV-A irradiation activates Nrf2-regulated antioxidant defense and induces p53/caspase3-dependent apoptosis in corneal endothelial cellsUV-A activates Nrf2 and induces p53 in corneal endothelial cells[J].Investigative Ophthalmology & Visual Science, 2016, 57(4): 2319~2327. [19] Ye S, Zhang D, Cheng F, et al. Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal[J].Cell Sci, 2016, 129(2): 269~276. [20] Yue X, Cao D, Lan F M, et al. MiR-301a is activated by the Wnt/β-catenin pathway and promotes glioma cell invasion by suppressing SEPT7[J].Neuro-oncology, 2016, 18(9): 1288~1296. [21] Shi J, Jiang X, Yu Z, et al. ZNRF3 contributes to the growth of lung carcinoma via inhibiting Wnt/β-catenin pathway and is regulated by miR-93[J].Tumor Biology, 2016, 37(3):306.
2017, Vol. 23 
冀公网安备13080202000677号