Abstract：Objective: To explore the potential role of miR-543 in regulating the radiosensitivity of NSCLC cells. Methods: The expression of miR-543 was detected by qRT-PCR; CCK8,colony formation assay and flow cytometry were used to explore the effect of miR-543 on the biological function of NSCLC cells.The expression of proteins related to AMPK-ACC signaling pathway was detected by WB.Reversion experiments and radiation treatment experiments were used to further verify the effect of miR-543 on the malignant progression and radiotherapy resistance of NSCLC cells through the AMPK-ACC signaling pathway. Results: miR-53 was up-regulated in NSCLC tissues and cells.Silencing miR-53 can inhibit the proliferation ability of NSCLC cells,promote cell cycle arrest and apoptosis,and weaken radiotherapy resistance.In addition,miR-53 is involved in the regulation of AMPK-ACC signaling pathway,and the use of compound C (an inhibitor of AMPK-ACC signaling pathway) can attenuate the effect of miR-53 silencing on NSCLC cell behavior and radiosensitivity. Conclusion: The results of this study suggest that miR-53 can enhance the radiotherapy resistance mechanism in NSCLC cells by regulating the AMPK-ACC signaling pathway,suggesting that targeting miR-53 may be a potential therapeutic target to improve the radiotherapy sensitivity in NSCLC.
储祥健, 曹海燕, 戴美云. 基于AMPK-ACC信号通路探究miR-543对肺癌细胞放疗敏感性的机制研究[J]. 河北医学, 2023, 29(7): 1068-1073.
CHU Xiangjian, CAO Haiyan, DAI Meiyun. The Mechanism of miR-543 on Radiosensitivity in Lung Cancer Cells Based on AMPK-ACC Signaling Pathway. HeBei Med, 2023, 29(7): 1068-1073.
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2023, Vol. 29 
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