Abstract：Objective: To explore the killing effect of 5-fluorouracil cell vesicles on colorectal cancer cell line HCT116. Methods: The HCT116 group, cell vesicles group (EVs suspension 5mL, 106 cells/mL), 5-fluorouracil group (5-fluorouracil 5mL, 30μg/mL), and 5-fluorouracil drug-loaded vesicles group (EVs suspension 5mL, 106 cells/mL+5-fluorouracil 5mL, 30μg/mL) were set up, and 6 parallel samples of each well of the above groups were incubated for 72h. After incubation, the levels of cell proliferation, invasion and apoptosis were determined, and the levels of miR-128 and PIK3 were determined by RT-PCR and western blotting. Results: The OD value, survival rate, number of monoclonal formation, number of membrane penetration, migration distance, apoptosis rate, miR-128 level, PI3K mRNA and protein levels in cell vesicles group were not significantly different from those in HCT116 group(P>0.05). OD value, survival rate, number of monoclonal formation, number of membrane penetration, migration distance, PI3KmRNA and protein levels in 5-fluorouracil group and 5-fluorouracil drug-loaded vesicles group were significantly lower than those in HCT116 group and cell vesicles group(P<0.05), and the apoptosis rate and the level of miR-128 were significantly higher than those in HCT116 group and cell vesicle group (P<0.05). OD value, survival rate, number of monoclonal formation, number of membrane penetration, migration distance, PI3K mRNA and protein levels in 5-fluorouracil drug-loaded vesicles group were significantly lower than those in 5-fluorouracil group, and the apoptosis rate and miR-128 level were significantly higher than those in 5-fluorouracil group(P<0.05). Conclusion: Extracellular vesicles loaded with 5-fluorouracil can significantly inhibit the proliferation and invasion level of colorectal cancer cell line HCT116, and enhance its apoptosis level, which may be related to the mechanism that extracellular vesicles loaded with 5-fluorouracil can promote the high expression of miR-128 and low expression of PI3K in colorectal cancer cell line HCT116.
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2023, Vol. 29 
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