槲皮素对口腔鳞癌细胞增殖凋亡等细胞生物学行为及p38/JNK MAPK信号通路的影响

doi:10.3969/j.issn.1006-6233.2023.04.02

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摘要 目的: 探讨槲皮素对口腔鳞癌细胞增殖、凋亡、迁移、侵袭及p38/JNK MAPK信号通路的影响。方法: 以体外培养的口腔鳞癌Tac8113细胞为研究对象,分别设置空白对照组,槲皮素低剂量组(25μmoL/L)、高剂量组(50μmoL/L)及阳性对照组(顺铂4μg/mL)。CCK-8法检测槲皮素对口腔鳞癌Tac8113细胞增殖的影响;流式细胞仪检测槲皮素对口腔鳞癌Tac8113细胞凋亡的影响;Transwell实验检测槲皮素对口腔鳞癌Tac8113细胞迁移、侵袭的影响;WB法检测p38/JNK MAPK信号通路相关蛋白表达。结果: 与空白对照组相比,槲皮素低、高剂量组及阳性对照组Tac8113细胞增殖活力、迁移数及侵袭数均下降,凋亡率增加(P<0.05);与槲皮素低剂量组相比,槲皮素高剂量组及阳性对照组Tac8113细胞增殖活力、迁移数及侵袭数均下降,凋亡率增加(P<0.05),呈现剂量依赖性;槲皮素高剂量组及阳性对照组Tac8113细胞增殖活力、迁移数、侵袭数及凋亡率差异无统计学意义(P>0.05)。与空白对照组相比,槲皮素低、高剂量组p-p38、p-JNK 及Caspase-3蛋白表达增加(P<0.05);与槲皮素低剂量组相比,槲皮素高剂量组p-p38、p-JNK 及Caspase-3蛋白表达增加(P<0.05),呈现剂量依赖性;空白对照组、槲皮素低剂量组、槲皮素高剂量组、阳性对照组p38、JNK蛋白表达差异不显著(P>0.05)。结论: 槲皮素可以降低口腔鳞癌Tac8113细胞的增殖活力、迁移和侵袭数,诱导Tac8113细胞凋亡,这可能是通过激活p38/JNK MAPK信号通路实现的。

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	胡硕红
	郑学彬
	李富杰

关键词 ：槲皮素, 口腔鳞癌细胞, 细胞生物学行为, p38/JNKMAPK信号通路

Abstract：Objective: To investigate the effect of quercetin on the proliferation, apoptosis, migration, invasion and p38/JNK MAPK signaling pathway of oral squamous cell carcinoma cells. Methods: Oral squamous cell carcinoma Tac8113 cells cultured in vitro were used as research subjects, and blank control group, low-dose quercetin group (25μmol/L), high-dose quercetin group (50μmol/L) and positive control group (cisplatin 4μg/mL) were set respectively. The effect of quercetin on the proliferation of oral squamous cell carcinoma Tac8113 cells was detected by CCK-8 method; the effect of quercetin on the apoptosis of oral squamous cell carcinoma Tac8113 cells was detected by flow cytometry; the migration and invasion of oral squamous cell carcinoma Tac8113 cells was detected by Transwell assay; WB method was used to detect the expression of p38/JNK MAPK signaling pathway-related proteins. Results: Compared with the blank control group, the proliferation activity, migration number and invasion number of Tac8113 cells in the low-dose and high-dose quercetin groups and the positive control group decreased, and the apoptosis rate increased (P<0.05). Compared with Tac8113 cells in the high-dose quercetin group and the positive control group, the proliferation activity, the number of migration and the number of invasion decreased, and the apoptosis rate increased (P<0.05), in a dose-dependent manner; There was no significant difference in the proliferation activity, migration number, invasion number and apoptosis rate of Tac8113 cells between the high-dose quercetin group and the positive control group. (P>0.05). Compared with the blank control group, the expressions of p-p38, p-JNK and Caspase-3 in the low-dose and high-dose quercetin groups were increased (P<0.05); compared with the low-dose quercetin group, the protein expressions of p-p38, p-JNK and Caspase-3 in the high-dose quercetin group were increased (P<0.05), in a dose-dependent manner; The differences in p38 and JNK protein expression among the blank control group, quercetin low dose group, quercetin high dose group, and positive control group were not significant (P>0.05). Conclusion: Quercetin can reduce the proliferation activity, migration and invasion number of oral squamous cell carcinoma Tac8113 cells, and induce Tac8113 cell apoptosis, which may be achieved by activating the p38/JNK MAPK signaling pathway.

Key words： Quercetin Oral squamous cell carcinoma cells Cell biological behavior p38/JNK MAPK signaling pathway

基金资助:海南省卫生计生行业科研项目,(编号:18A200152)

引用本文:

胡硕红, 郑学彬, 李富杰. 槲皮素对口腔鳞癌细胞增殖凋亡等细胞生物学行为及p38/JNK MAPK信号通路的影响[J]. 河北医学, 2023, 29(4): 535-540.
HU Shuohong, ZHENG Xuebin, LI Fujie. Effects of Quercetin on Cell Biological Behaviors Including Proliferation Apoptosis and p38/JNK MAPK Signaling Pathway in Oral Squamous Carcinoma Cells. HeBei Med, 2023, 29(4): 535-540.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2023.04.02 或 http://www.hbyxzzs.cn/CN/Y2023/V29/I4/535

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