Abstract：Objective: To investigate the regulation of long non-coding RNA nuclear-enriched abundant transcript 1 (lncRNA NEAT1) on the miR-506-5p/cofilin-2 (CFL2) axis, and its influences on the biological behaviors of gastric cancer cells such as proliferation, apoptosis, migration, and invasion. Methods: BGC-823 cells were cultured in vitro, and lncRNA NEAT1 low expression vector (si-NEAT1) and miR-506-5p inhibitor (miR-506-5p inhibitor) were transfected into BGC-823 cells, which were divided into the si-NC group, si-NEAT1 group, inhibitor NC group, miR-506-5p inhibitor NC group, si-NEAT1+miR-506-5p inhibitor NC group, and si-NEAT1+miR-506-5p inhibitor group. RT-qPCR was performed to determine the expression of lncRNA NEAT1 and miR-506-5p to determine the transfection efficiency. CCK-8 assay was performed to determine proliferation. Flow cytometry was performed to determine apoptosis; a Transwell assay was performed to determine migration and invasion. Western blot was performed to determine the protein expressions of CFL2 and cyclin-dependent kinase 4 (CDK4). Dual-luciferase validation assay was performed to validate the targeting relationship of lncRNA NEAT1, CFL2, and miR-506-5p; nude mouse tumor-bearing experiments were carried out to further verify the targeted regulation of lncRNA NEAT1, miR-506-5p, and CFL2. Results: Compared with the si-NC group, cell proliferation, invasion ability, and CFL2 expression in the si-NEAT1 group were decreased, while apoptosis rate and miR-506-5p expression were increased (P<0.05). Compared with the inhibitor NC group, cell proliferation, invasion ability, and CFL2 expression were increased in the miR-506-5p inhibitor group, while apoptosis rate and miR-506-5p expression were decreased (P<0.05). LncRNA NEAT1 could target and regulate the expression of miR-506-5p, and CFL2 was the target gene of miR-506-5p. Knockdown of miR-506-5p could weaken the anti-proliferation, anti-invasion, and pro-apoptosis effects of lncRNA NEAT1 low expression (P<0.05). miR-506-5p inhibitor attenuated the anti-graft tumor growth effect of lncRNA NEAT1 low expression (P<0.05). Overexpression of CFL2 attenuated the anti-graft tumor growth effect of overexpression of miR-506-5p (P<0.05). Conclusion: LncRNA NEAT1 can affect the biological behaviors such as proliferation, invasion, and apoptosis of human gastric cancer cells by targeting and regulating the miR-506-5p/CFL2 axis.
闫丹措, 逯艳艳, 刘芝兰, 马颖才, 丹朱永吉, 薛晓红. LncRNA NEAT1通过调节miR-506-5p/CFL2轴对人胃癌细胞增殖凋亡和侵袭的影响[J]. 河北医学, 2023, 29(3): 412-417.
YAN Dancuo, LU Yanyan, LIU Zhilan, et al. Influences of LncRNA NEAT1 on Proliferation Apoptosis and Invasion of Human Gastric Cancer Cells by Regulating the miR-506-5p/CFL2 Axis. HeBei Med, 2023, 29(3): 412-417.
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2023, Vol. 29 
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