Abstract：Objective: To investigate the expression and clinical significance of CD68-labeled tumor-associated macrophages (CD68-TAMS) and CD105-labeled microvascular density (CD105-MVD) in laryngeal squamous cell carcinoma. Methods: The paraffin specimens of 40 cases of laryngeal squamous cell carcinoma confirmed by surgical resection and pathological examination were retrospectively studied, and 20 cases of adjacent normal tissues (not infiltrated by laryngeal carcinoma cells) were used as control group. Immunohistochemical SP method was used to detect the protein expression of CD68-Tams and CD105 and microvessel density MVD. Kaplan-meier method was used to analyze the relationship between the expression of CD68-Tams and CD105-MVD and the prognosis of patients. Results: The positive expression rate of CD68-TAMS in laryngeal cancer group (77.5%, 31/40) was significantly higher than that in control group (20.00%, 2/20), and the difference was statistically significant (P<0.05). The positive expression rate of CD105-MVD in laryngeal carcinoma group (72.50%, 31/40) was significantly higher than that in control group (30.0%, 6/20) (P<0.05). The expression of CD68-Tams was correlated with lymph node metastasis, tumor differentiation and TNM stage (P<0.05), and the expression of CD105-MVD was correlated with lymph node metastasis and tumor differentiation (P<0.05). The 3-year survival rate of patients with positive CD68-Tams expression was lower than that of patients with low CD68-TAMS expression (P<0.05). The 3-year survival rate of patients with positive CD105-MVD expression was lower than that of patients with low CD105-MVD expression (P<0.05). Cox regression analysis showed that positive CD68-TAMs, positive CD105-MVD, tumor stage and lymph node metastasis were independent influencing factors for poor prognosis of laryngeal cancer (P<0.05). Conclusion: The expression of CD68-TAMs and CD105-MVD in laryngeal cancer tissues indicates that they promote tumor angiogenesis and tumor development, and can be used as markers for the diagnosis of laryngeal squamous cell carcinoma. Positive CD68-TAMs, positive CD105-MVD, tumor staging and lymph node metastasis in laryngeal cancer tissues are closely related to prognosis.It can be used as an independent evaluation indicator of poor prognosis of laryngeal cancer.
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2023, Vol. 29 
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