Abstract：Objective: To investigate the targeting relationship between miR-24-3p and bone morphogenetic protein 8B (BMP8B), and to analyze their effects on osteoblast function. Methods: Human periodontal ligament stem cells (hPDLSCs) were isolated and cultured in vitro and induced to differentiate into osteoblasts. The osteoblasts were identified by alkaline phosphatase (ALP) staining and alizarin red staining; the osteoblasts were divided into blank control group, negative control group (inhibitor-NC group), silencing miR-24-3p expression group (miR-24-3p-inhibitor group), and co-transfection group (miR-24-3p-inhibitor +BMP8B-siRNA group). Real-time fluorescent quantitative PCR method was used to detect the levels of miR-24-3p and BMP8B mRNA; CCK-8 method and flow cytometer were used to detect the proliferation and apoptosis of bone cells in each component; ALP activity detection kit was used to detect ALP activity; Western blotting was used to detect the levels of BMP8B protein and functional activity-related proteins (PICP, BGP, OPN) in each component of bone cells. The TargetScan database and the dual luciferase reporter gene experiment were used to predict and verify the targeting relationship between miR-24-3p and BMP8B respectively. Results: Compared with the blank control group and inhibitor-NC group, the osteoblast proliferation inhibition rate and apoptosis rate in the miR-24-3p-inhibitor group were decreased, ALP activity and expression of PICP, BGP, OPN were increased (P<0.05); compared with miR-24-3p-inhibitor group, the osteoblast proliferation inhibition rate and apoptosis rate in the miR-24-3p-inhibitor+BMP8B-siRNA group were increased, ALP activity and expression of PICP, BGP, OPN were decreased (P<0.05). The dual luciferase reporter gene experiment confirmed that miR-24-3p targeted regulation of BMP8B. Conclusion: Inhibition of miR-24-3p may promote the proliferation and differentiation of osteoblasts and inhibit cell apoptosis by targeting up-regulation of BMP8B expression.
王建琪, 李召宝, 吴帅楠, 郭香君, 曹素敏, 林秀雅, 褚亚辉. miR-24-3p靶向BMP8B对成骨细胞功能影响的实验研究[J]. 河北医学, 2023, 29(1): 59-65.
WANG jianqi, LI Zhaobao, WU Shuanan, et al. Experimental Study on the Effect of miR-24-3p Targeting BMP8B on the Function of Osteoblasts. HeBei Med, 2023, 29(1): 59-65.
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2023, Vol. 29 
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