Abstract：Objective: To investigate the expression of serum microRNA-181a-5p (miR-181a-5p) in patients with acute pancreatitis (AP) and the relationship with T helper cell 17/T regulatory cell (Th17/Treg) immune homeostasis. Methods: 110 patients with AP admitted to our hospital from January 2020 to December 2021 (AP group) were selected and divided into the mild AP group (n=41), the moderately severe AP group (n=37)and the severe AP group (n=32) according to the severity of their conditions, and 58 healthy people with physical examination (control group) were also selected for the same period. Peripheral blood interleukin-6 (IL-6), IL-10, IL-17 and transforming growth factor-β1 (TGF-β1) levels were measured by enzyme-linked immunosorbent assay, peripheral blood Th17 and Treg percentages were measured by flow cytometry, and serum miR-181a-5p expression was measured by qPCR. Correlation of serum miR-181a-5p expression with Th17/Treg in AP patients was analyzed using Pearson/Spearman correlation. Results: Compared with the control group, serum miR-181a-5p expression and peripheral blood Treg percentage decreased, and Th17 percentage and Th17/Treg increased in AP group (all P<0.001). Compared with the control group, the peripheral blood levels of IL-6 and IL-17 in the AP group were increased, and the levels of IL-10 and TGF-β1 were reduced (all P<0.001). Serum miR-181a-5p expression and peripheral blood IL-10, TGF-β1, and Treg percentage were sequentially lower and peripheral blood IL-6, IL-17, Th17 percentage, and Th17/Treg were sequentially higher in the mild, moderately severe, and severe AP groups (all P<0.001). Pearson/Spearman correlation analysis showed that serum miR-181a-5p expression in AP patients was negatively correlated with IL-6, IL-17, Th17, and Th17/Treg (r/rs=-0.688, -0.652, -0.782, -0.825, all P<0.001) and positively correlated with IL-10, TGF-β1, and Treg were positively correlated (r/rs=0.743, 0.667, 0.739, all P<0.001). Conclusion: Serum miR-181a-5p is lowly expressed in AP patients with Th17/Treg immune imbalance. miR-181a-5p may be involved in the development of AP through Th17/Treg immune imbalance.
[1] 中华医学会外科学分会胰腺外科学组.中国急性胰腺炎诊治指南(2021)[J].中华消化外科杂志,2021,20(7):730-739. [2] 中国医疗保健国际交流促进会急诊医学分会,脓毒症预防与阻断联盟.重症急性胰腺炎预防与阻断急诊专家共识[J].中国急救医学,2022,42(5):369-379. [3] 郑智,曲元旭,丁乙轩,等.急性胰腺炎发病机制的研究进展[J].中华肝胆外科杂志,2021,27(2):152-155. [4] 张涛,李智伟,徐辉.Treg/Th17细胞免疫平衡紊乱与急性胰腺炎疾病进展相关性研究[J].中国普外基础与临床杂志,2022,29(7):892-896. [5] Zhou W,Dong S,Chen Z,et al.New challenges for microRNAs in acute pancreatitis:progress and treatment[J].Transl Med,2022,20(1):192. [6] An F,Zhan Q,Xia M,et al.From moderately severe to severe hypertriglyceridemia induced acute pancreatitis:circulating miRNAs play role as potential biomarkers[J].PLoS One,2014,9(11):e111058. [7] 中华医学会,中华医学会杂志社,中华医学会消化病学分会,等.急性胰腺炎基层诊疗指南(实践版·2019)[J].中华全科医师杂志,2019,18(9):827-831. [8] 中华医学会急诊分会,京津冀急诊急救联盟,北京医学会急诊分会,等.急性胰腺炎急诊诊断及治疗专家共识[J].中华急诊医学杂志,2021,30(2):161-172. [9] 赵先林,朱世峰,康鸿鑫,等.急性胰腺炎与表观遗传调控机制[J].临床肝胆病杂志,2020,36(1):227-230. [10] Sun C,Rong Y,Yang Z,et al.Construction of dual-target recognition-based specific microRNA detection method for acute pancreatitis analysis[J].Appl Biochem Biotechnol,2022,194(7):3136-3144. [11] Wu Z,Zhang Z,Wang Z,et al.MiR-181a-5p alleviates the inflammatory response of PC12 cells by inhibiting high-mobility group box-1 protein expression[J].World Neurosurg,2022,162(6):427-435. [12] Liu J,Niu Z,Zhang R,et al.MALAT1 shuttled by extracellular vesicles promotes M1 polarization of macrophages to induce acute pancreatitis via miR-181a-5p/HMGB1 axis[J].Cell Mol Med,2021,25(19):9241-9254. [13] 赵萌,金立方,李小丰,等.原发免疫性血小板减少症miRNA181a-5p表达与Treg/Th17失衡关系的研究[J].中国实验血液学杂志,2022,30(1):237-243.
2023, Vol. 29 
冀公网安备13080202000677号