Abstract：Objective: To investigate the influences of erythropoietin (EPO) on the proliferation and apoptosis of neural stem cells induced by hypoxia by regulating hypoxia-inducible factor-1α (HIF-1α)/extracellular signal-regulated kinase (ERK) signaling pathway. Methods: Neural stem cells were divided into NC group, Model group, low-dose EPO group (EPO-L group, 5 U/mL), and high-dose EPO group (EPO-H group, 25 U/mL) , YC-1 group, and EPO-H+YC-1 (HIF-1α inhibitor) group (25 U/mL +10μmol/L). Western blot was used to detect the protein expression of Cyclin D1, Bcl-2/adenovirus interfering protein 3 (BNIP3), Cleaved Caspase-3, HIF-1α, and p-ERK1/2. Results: Compared with NC group, the proportion of Brd U positive cells, and the protein expression of Cyclin D1, HIF-1α and p-ERK1/2 in Model group decreased, and the apoptosis rate, the protein expression of BNIP3 and Cleaved Caspase-3 increased (P<0.05); compared with the Model group, the proportion of Brd U positive cells, and the protein expression of Cyclin D1, HIF-1α and p-ERK1/2 in EPO-L group and EPO-H group increased, and the apoptosis rate, the protein expression of BNIP3 and Cleaved Caspase-3 decreased (P<0.05), the corresponding indexes in YC-1 group were opposite to those above (P<0.05); YC-1 weakened the promotion of high dose EPO on the proliferation of neural stem cells induced by hypoxia and the inhibition of apoptosis. Conclusion: EPO may promote hypoxia-induced neural stem cell proliferation and inhibit cell apoptosis by activating the HIF-1α/ERK pathway.
罗聪, 钟崛, 沈坭烺, 张爱华, 吴盛兰, 陈舒. 促红细胞生成素对低氧诱导的神经干细胞增殖凋亡的影响[J]. 河北医学, 2023, 29(1): 49-53.
LUO Cong, et al. Effect of Erythropoietin on Proliferation and Apoptosis of Neural Stem Cells Induced by Hypoxia. HeBei Med, 2023, 29(1): 49-53.
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2023, Vol. 29 
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