Abstract：Objective: To study the mechanism of hypoxic exercise energy metabolism in obese rats . Methods: Eighty male SD rats were randomly divided into normal diet control group (10 rats) and high-fat diet model group (70 rats), and the model group rats were induced with high-fat diet for 7 weeks to construct an obese rat model. 60 obese rats with successful modeling were randomly divided into normoxic quiet group (group A), normoxic exercise group (group AE), 16. 3% hypoxic quiet group (group B), 16. 3% hypoxic exercise group (group BE), 13. 3% hypoxic quiet group (group C), and 13. 3% hypoxic exercise group (group CE). group), 13. 3% hypoxic quiet group (group C), 13. 3% hypoxic exercise group (group CE), 10 rats in each group, continued to be fed with high-fat diet, and the rats in the exercise group were trained with running platform endurance, and body length and body weight were measured, and Lee's index was calculated. Blood glucose (BG) and lipid quadruple (BG, TC, LDL-C, TG) were detected; oxygen consumption and energy expenditure after exercise were detected using rat metabolic system; PPAR-α and PGC-1α protein expression in skeletal muscle tissue were detected. Results: After 7 weeks of high-fat dietary feeding, the rats' body weight increased significantly and exceeded 20% of the body weight of the control rats, and blood glucose, TC, TG, and LDL-C contents increased significantly (P<0. 01, P<0. 05), suggesting that the nutritional obesity rat model was successfully established. After 8 weeks of intervention with hypoxia and endurance exercise, the body weight gain, TC, TG, LDL-C, and blood glucose of rats in AE, BE, C, and CE groups were significantly decreased (P<0. 05, P<0. 01), and HDL-C of rats in BE and CE groups were significantly increased (P<0. 01) compared with group A. Compared with group AE, the body weight gain, TC, TG, LDL-C and blood glucose of rats in groups BE and CE were significantly lower (P<0. 01), and the HDL-C of rats in groups BE and CE was significantly higher (P<0. 01); compared with group A, the immediate oxygen consumption and energy consumption of rats in the AE, B, BE, C and CE groups showed an increasing trend (P<0. 01, P<0. 05); compared with group AE, groups BE and CE showed an increase in oxygen consumption and energy consumption immediately after exercise (P<0. 05); compared with group A, the expression of PPAR-α and PGC-1α protein in groups BE, C and CE was significantly increased (P<0. 05, P<0. 01); compared with group AE, the expression of PPAR-α and PGC-1α protein in the BE, C and CE rats was significantly increased (P<0. 05, P<0. 01). Protein expression was significantly increased in BE, C and CE rats compared with group AE (P<0. 05, P<0. 01). Conclusion: Hypoxic exercise may enhance the expression of PGC-1α and PPAR-α proteins in skeletal muscle and thus improve the energy metabolism level in obese rats.
郜琨, 杨历新, 王叶, 李正, 李小鸾. 基于PPAR-α/PGC-1α途径探讨低氧减重对肥胖大鼠能量代谢的影响[J]. 河北医学, 2022, 28(12): 1978-1984.
GAO Kun, YANG Lixin, WANG Ye, et al. The Effect of Hypoxia Exercise on Energy Metabolism in Obese Rats Based on PPAR-α/PGC-1α Pathway. HeBei Med, 2022, 28(12): 1978-1984.
2022, Vol. 28 
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