Abstract：Objective: To investigate the effect of circPTK2 on the invasion and migration of esophageal cancer (EC) cells by regulating miR-619-5p.Methods: QRT-PCR was performed to measure the expressions of circPTK2 and miR-619-5p in EC tissues and cell lines (OE19, KYSE-450, KYSE-520, TE-13). TE-13 cells were randomly separated into control group, si-NC, si-circPTK2 group, miR-NC group, miR-619-5p group, si-circPTK2+anti-NC group and si-circPTK2+anti-miR-619- 5p group. Cell viability analysis was performed with Cell Counting Box-8 (CCK-8); the cell invasion and migration ability were measured by Transwell assay; the expression of epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin, N-cadherin, Vimentin) was measureed by western blot. The interaction between circPTK2 and miR-619-5p was verified by dual-luciferase reporter gene assay and RNA pull-down experiment. The role of circPTK2 in vivo was investigated using xenograft experiments. Results: The expression of circPTK2 was up-regulated in EC tissues and cells, and the expression of miR-619-5p was down-regulated (P<0.05). Downregulation of circPTK2 expression or overexpression of miR-619-5p inhibited cell growth, invasion, migration and EMT (P<0.05). MiR-619-5p was a target of circPTK2. Inhibition of miR-619-5p reversed the effects of circPTK2 downregulation on EC cell proliferation, invasion, migration and EMT (P<0.05). In addition, inhibition of miR-619-5p expression could partially restore the effect of downregulation of circPTK2 on tumor growth (P<0.05). Conclusion: CircPTK2 promotes the growth, invasion and migration of EC cells through sponge adsorption of miR-619-5p, circPTK2 may become a potential therapeutic target for EC metastasis and is a promising biomarker for predicting EC cell metastasis.
付堂清, 李文忠, 罗仕云, 师路, 杜镇鸿. circPTK2调控miR-619-5p对食道癌细胞侵袭迁移的影响[J]. 河北医学, 2022, 28(11): 1778-1784.
FU Tangqing, LI Wenzhong, LUO Shiyun, et al. Effect of circPTK2 Regulation of miR-619-5p on Invasion and Migration of Esophageal Cancer Cells. HeBei Med, 2022, 28(11): 1778-1784.
[1] Domper Arnal MJ,Ferrandez Arenas a,Lanas Arbeloa a.Esophageal cancer: risk factors,screening and endoscopic treatment in Western and Eastern countries[J].World Gastroenterol,2015,21(26):7933-7943. [2] Kurtom S,Kaplan BJ.Esophagus and gastrointestinal junction tumors[J].Surg Clin North Am,2020,100(3):507-521. [3] Qin J,Peng Y,Chen W,et al.Comparative study of esophagectomy,endoscopic therapy and radiotherapy for cT1N0M0 esophageal cancer in elderly patients: A SEER database analysis[J].Thorac Cancer,2019,10(7):1511-1520. [4] Park R,Williamson S,Kasi A,et al.Immune therapeutics in the treatment of advanced gastric and esophageal cancer[J].Anticancer Res,2018,38(10):5569-5580. [5] Li XY,Yang B,Sun Y,et al.Effect of hsa_circ_RNA0023397 regulation of miR-106b expression on proliferation and apoptosis of esophageal cancer cells[J].Eur Rev Med Pharmacol Sci,2020,24(17):8855-8862. [6] Zhang Z,Lin W,Gao L,et al.Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway[J].Biosci Rep,2019,39(5):20182377-20182387. [7] Yang H,Li X,Meng Q,et al.CircPTK2 (hsa_circ_0005273) as a novel therapeutic target for metastatic colorectal cancer[J].Mol Cancer,2020,19(1):13-28. [8] Wang Y,Wu Y,Xie S.CircPTK2 inhibits cell cisplatin (CDDP) resistance by targeting miR-942/TRIM16 axis in non-small cell lung cancer (NSCLC)[J].Bioengineered,2022,13(2):3651-3664. [9] Song A,Wu Y,Chu W,et al.Involvement of miR-619-5p in resistance to cisplatin by regulating ATXN3 in oral squamous cell carcinoma[J].Int Biol Sci,2021,17(2):430-447. [10] Chen W,Schuman E.Circular RNAs in brain and other tissues: a functional enigma[J].Trends Neurosci,2016,39(9):597-604. [11] Zhang LW,Wang B,Yang JX,et al.Circ-PRMT5 stimulates migration in esophageal cancer by binding miR-203[J].Eur Rev Med Pharmacol Sci,2020,24(19):9965-9972. [12] Yu D,Zhang C.Circular RNA PTK2 accelerates cell proliferation and inhibits cell apoptosis in gastric carcinoma via miR-139-3p[J].Dig Dis Sci,2021,66(5):1499-1509. [13] Mu M,Niu W,Zhang X,et al.LncRNA BCYRN1 inhibits glioma tumorigenesis by competitively binding with miR-619-5p to regulate CUEDC2 expression and the PTEN/AKT/p21 pathway[J].Oncogene,2020,39(45):6879-6892. [14] Xie ZF,Li HT,Xie SH,et al.Circular RNA hsa_circ_0006168 contributes to cell proliferation,migration and invasion in esophageal cancer by regulating miR-384/RBBP7 axis via activation of S6K/S6 pathway[J].Eur Rev Med Pharmacol Sci,2020,24(1):151-163. [15] Wang J,Chen Y,Wu R,et al.Circular RNA hsa_circ_0000554 promotes progression and elevates radioresistance through the miR-485-5p/fermitin family members 1 axis in esophageal cancer[J].Anticancer Drugs,2021,32(4):405-416.
2022, Vol. 28 
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