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河北医学  2022, Vol. 28 Issue (11): 1774-1777    DOI: 10.3969/j.issn.1006-6233.2022.11.03
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circ_0000615靶向调控miR-142-3p对骨肉瘤细胞增殖迁移侵袭的影响
李贺伟, 阮锋
华中科技大学同济医学院附属梨园医院, 湖北 武汉 430077
Effect of circ_0000615 on Proliferation Migration and Invasion of Osteosarcoma Cells by Targeting miR-142-3p
LI Hewei, RUAN Feng
Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei Wuhan 430077, China
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摘要 目的: 探讨circ_0000615对骨肉瘤细胞增殖、迁移、侵袭的影响及分子机制。方法: 选取60例骨肉瘤患者的骨肉瘤组织及瘤旁组织,实时荧光定量PCR(RT-qPCR)检测circ_0000615和miR-142-3p表达水平;将骨肉瘤细胞U2OS分为si-circ_0000615组、si-NC组、miR-142-3p组、miR-NC组、si-circ_0000615+anti-miR-142-3p组、si-circ_0000615+anti-miR-NC组;四甲基偶氮唑盐比色法(MTT)检测细胞抑制率;克隆形成实验检测细胞克隆数;Transwell法检测U2OS细胞迁移和侵袭数;蛋白质印迹法检测蛋白表达;双荧光素酶报告实验检测circ_0000615和miR-142-3p的靶向关系。结果: 与瘤旁组织相比,骨肉瘤组织中circ_0000615表达水平升高,miR-142-3p表达水平降低(P<0.05)。沉默circ_0000615或过表达miR-142-3p后,U2OS细胞抑制率、E-cadherin表达水平升高,N-cadherin表达水平降低,U2OS细胞克隆数、迁移侵袭数减少(P<0.05)。circ_0000615靶向调控miR-142-3p;抑制miR-142-3p可减轻circ_0000615对U2OS细胞增殖、迁移和侵袭的抑制作用。结论: 沉默circ_0000615可通过靶向调控miR-142-3p抑制骨肉瘤细胞的增殖、迁移和侵袭。
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李贺伟
阮锋
关键词 circ_0000615miR-142-3p骨肉瘤增 殖迁 移侵 袭    
AbstractObjective: To explore the effect of circ_0000615 on the proliferation, migration and invasion of osteosarcoma cells and its molecular mechanism.Methods: The osteosarcoma tissues and adjacent tissues of 60 patients with osteosarcoma were selected, and real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression levels of circ_0000615 and miR-142-3p; the osteosarcoma cells U2OS were divided into si-circ_0000615 group, si-NC group, miR-142-3p group, miR-NC group, si-circ_0000615+anti-miR-142-3p group, si-circ_0000615+anti-miR-NC group; tetramethylazolium salt colorimetric method (MTT) to detect cell inhibition rate; clone formation experiment to detect cell clone number; Transwell method to detect U2OS cell migration and invasion numbers; Western blot method to detect protein expression; dual luciferase reporter experiment to detect the targeting relationship between circ_0000615 and miR-142-3p. Results: Compared with the adjacent tissues, the expression level of circ_0000615 in osteosarcoma tissue was increased, and the expression level of miR-142-3p was decreased (P<0.05). After silencing circ_0000615 or overexpressing miR-142-3p, the inhibition rate of U2OS cells was increased, the number of clones, migration and invasion of U2OS cells were decreased, the expression level of E-cadherin was increased, and the expression level of N-cadherin was decreased (P<0.05). circ_0000615 targets and regulates miR-142-3p; inhibition of miR-142-3p can reduce the inhibitory effect of circ_0000615 on U2OS cell proliferation, migration and invasion. Conclusion: Silencing circ_0000615 can inhibit the proliferation, migration and invasion of osteosarcoma cells through targeted regulation of miR-142-3p.
Key wordscirc_0000615    miR-142-3p    Osteosarcoma    Proliferation    Migration    Invasion
    
基金资助:湖北省武汉市卫生健康委员会资助项目,(编号:武卫通【2019】87号)
通讯作者: 阮锋   
引用本文:   
李贺伟, 阮锋. circ_0000615靶向调控miR-142-3p对骨肉瘤细胞增殖迁移侵袭的影响[J]. 河北医学, 2022, 28(11): 1774-1777.
LI Hewei, RUAN Feng. Effect of circ_0000615 on Proliferation Migration and Invasion of Osteosarcoma Cells by Targeting miR-142-3p. HeBei Med, 2022, 28(11): 1774-1777.
链接本文:  
http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.11.03     或     http://www.hbyxzzs.cn/CN/Y2022/V28/I11/1774
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