结直肠癌组织中lncRNA TUSC7与miR-1270表达的相关性及临床病理意义

doi:10.3969/j.issn.1006-6233.2022.10.08

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摘要 目的: 探究结直肠癌(CRC)组织中长链非编码RNA(lncRNA)肿瘤抑制候选基因7(TUSC7)与微小RNA-1270(miR-1270)的相关性及二者与CRC患者预后的关系。方法: lnCAR数据库检索lncRNA TUSC7在CRC和正常结直肠组织中的表达情况。选取2018年6月至2019年1月本院收治的130例CRC患者为研究对象,收集手术切除的CRC和癌旁组织,检测CRC和癌旁组织中lncRNA TUSC7、miR-1270表达水平;分析CRC组织中lncRNA TUSC7表达水平与miR-1270相关性;分析CRC组织lncRNA TUSC7、miR-1270表达水平与CRC临床病理特征和预后关系。生物信息学网站预测lncRNA TUSC7与miR-1270的关系。结果: lnCAR数据库分析显示,lncRNA TUSC7在CRC组织中的表达水平低于正常结直肠组织(P<0.05);qRT-PCR结果显示,CRC组织中lncRNA TUSC7表达水平低于癌旁组织(P<0.05),miR-1270表达水平高于癌旁组织(P<0.05);CRC组织lncRNA TUSC7、miR-1270表达水平与分化程度、淋巴结转移、浸润深度、TNM分期相关(P<0.05);生物信息学分析显示,lncRNA TUSC7与miR-1270存在靶向结合位点;CRC组织中lncRNA TUSC7表达水平与miR-1270呈负相关(r=-0.578,P<0.05);lncRNA TUSC7低表达组、miR-1270高表达组CRC患者累积生存率低于lncRNA TUSC7高表达组、miR-1270低表达组(P<0.05)。结论: CRC组织中lncRNA TUSC7表达下调,miR-1270表达上调,二者呈负相关,为临床诊治CRC、评估CRC患者预后提供新标志物。

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关键词 ：微小RNA-1270, 结直肠癌, 长链非编码RNA肿瘤抑制候选基因7

Abstract：Objective: To investigate the correlation between long non-coding RNA (lncRNA) tumor suppressor candidate 7 (TUSC7) and microRNA-1270 (miR-1270) in colorectal cancer (CRC) tissues and their relationship with the prognosis of CRC patients. Methods: The lnCAR database was used to search the expression of lncRNA TUSC7 in CRC and normal colorectal tissues.A total of 130 CRC patients accepted by our hospital from June 2018 to January 2019 were regarded as the research subjects.The surgically resected CRC and paracancerous tissues were collected,and the expression levels of lncRNA TUSC7 and miR-1270 in CRC and paracancerous tissues were detected; the correlation between the expression level of lncRNA TUSC7 and miR-1270 in CRC tissues was analyzed; the relationship between the expression levels of lncRNA TUSC7 and miR-1270 in CRC tissues and the clinicopathological characteristics and prognosis of CRC were analyzed,Bioinformatics website was used to predict the relationship between lncRNA TUSC7 and miR-1270. Results: The lnCAR database analysis showed that the expression level of lncRNA TUSC7 in CRC tissue was lower than that in normal colorectal tissue (P<0.05); qRT-PCR results showed that the expression level of lncRNA TUSC7 in CRC tissue was lower than that in adjacent tissue (P<0.05),and the expression level of miR-1270 was higher than that in adjacent tissue (P<0.05); the expression levels of lncRNA TUSC7 and miR-1270 in CRC tissue were correlated with the degree of differentiation,lymph node metastasis,depth of invasion and TNM stage (P<0.05); bioinformatics analysis showed that lncRNA TUSC7 had a targeted binding site for miR-1270; the expression level of lncRNA TUSC7 in CRC tissue was negatively correlated with miR-1270 (r=-0.578,P<0.05); the cumulative survival rate of CRC patients in lncRNA TUSC7 low expression group and miR-1270 high expression group was lower than that in lncRNA TUSC7 high expression group and miR-1270 low expression group (P<0.05). Conclusion: The expression of lncRNA TUSC7 is down-regulated and the expression of miR-1270 is up-regulated in CRC tissue,and the two are negatively correlated,which provides a new marker for clinical diagnosis and treatment of CRC and evaluation of the prognosis of CRC patients.

Key words： MicroRNA-1270 Colorectal cancer Long non-coding RNA tumor suppressor candidate 7

基金资助:2021年度河北省医学科学研究课题计划,(编号:20210955)

引用本文:

姜辉, 任维聃, 刘桂伟, 冶浩鹏, 郭庆金, 耿学辰, 姜国胜. 结直肠癌组织中lncRNA TUSC7与miR-1270表达的相关性及临床病理意义[J]. 河北医学, 2022, 28(10): 1625-1630.
JIANG Hui, et al. Correlation and Clinicopathological Significance of lncRNA TUSC7 and miR-1270 Expression in Colorectal Cancer Tissues. HeBei Med, 2022, 28(10): 1625-1630.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.10.08 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I10/1625

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