Abstract：Objective: To culture a polyclonal antibody against the oprC protein of the outer membrane receptor of Acinetobacter baumannii (A.baumannii) and to evaluate its immune effect. Methods: The full gene sequence of oprC was queried on NCBI, and the full length of oprC was amplified using A.baumannii DNA as a template. The antibody titer was determined by enzyme-linked immunosorbent assay (ELISA), and the results showed that the potency of oprC antiserum was 1∶12800. The improvement of the survival rate, the number of bacteria in the blood, and the damage of multiple organ tissues of the mice challenged with oprC protein polyclonal antibody was detected. Results: The oprC polyclonal antibody could improve the survival rate of mice, reduce the load of bacteria (P<0.05), and relieve tissue damage to the lung, spleen, and kidney to some extent. Conclusion: This study confirmed that oprC recombinant protein can improve the survival rate of mice, reduce pathological damage, and achieve immune protection.
陈曦, 安娜, 张彬, 吴泳桦, 邓蔷, 胡冬. oprC蛋白多克隆抗体制备及其免疫效果研究[J]. 河北医学, 2022, 28(9): 1447-1451.
CHEN Xi, AN Na, ZHANG Bin, et al. Preparation of Polyclonal Antibody Against oprC Protein of Acinetobacter Baumannii and Its Immune Effect. HeBei Med, 2022, 28(9): 1447-1451.
[1] 李小霞,王燕,朱琳,等.55例耐碳青霉烯类革兰阴性菌新生儿败血症临床特征和病原菌分布及其耐药性[J].中华医院感染学杂志,2019,29(16):2500-2504. [2] 李笋,张卫军,陈大群,等.鲍曼不动杆菌外膜蛋白疫苗的制备及其保护作用评价[J].免疫学杂志,2020,36(2):93-101. [3] 王宇航,蔡芸.鲍曼不动杆菌对多黏菌素类抗菌药物耐药现状及机制研究进展[J].中国抗生素杂志,2019,44(9):1015-1019. [4] Pulido MR,Garcia-Quintanilla M,Pachon J,et al.A lipopolysaccharide-free outer membrane vesicle vaccine protects against Acinetobacter baumannii infection[J].Vaccine,2020,38(4):719-724. [5] Bielaszewska M,Daniel O,Karch H,et al.Dissemination of the blaCTX-M-15 gene among Enterobacteriaceae via outer membrane vesicles[J].Antimicrob Chemother,2020,75(9):2442-2451. [6] Martinez J,Razo-Gutierrez C,Le C,et al.Cerebrospinal fluid (CSF) augments metabolism and virulence expression factors in Acinetobacter baumannii[J].Sci Rep,2021,11(1):4737. [7] Liu B,Liu L.Molecular epidemiology and mechanisms of carbapenem-resistant acinetobacter baumannii isolates from ICU and respiratory department patients of a Chinese university hospital[J].Infect Drug Resist,2021,14(1):743-755. [8] Viale AM,Evans BA.Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii[J].Microb Genom,2020,6(6):e000381. [9] 安志远,丁文一.鲍曼不动杆菌外膜蛋白A慢病毒载体的构建及其对RAW264.7细胞NLRP3炎症小体的激活[J].中国病原生物学杂志,2019,14(2):146-150. [10] 蔺飞,余彬,袁明勇,等.鲍曼不动杆菌生物被膜形成与调控的研究进展[J].中国感染控制杂志,2019,18(12):1176-1183. [11] Skerniskyte J,Karazijaite E,Luciunaite A,et al.OmpA protein-deficient Acinetobacter baumannii outer membrane vesicles trigger reduced inflammatory response[J].Pathogens,2021,10(4):407. [12] Coines J,Acosta-Gutierrez S,Bodrenko I,et al.Glucose transport via the pseudomonad porin OprB:implications for the design of Trojan Horse anti-infectives[J].Phys Chem Chem Phys,2019,21(16):8457-8463. [13] Meshkat Z,Amini Y,Sadeghian H,et al.ISAba1/blaOXA-23-like family is the predominant cause of carbapenem resistance in Acinetobacter baumannii and Acinetobacter nosocomialis in Iran[J].Infect Genet Evol,2019,71(1):60-66. [14] 董嘉文,孙敏华,李林林,等.血清2型鸭疫里默氏杆菌铁载体受体蛋白突变体的构建及生物学特性研究[J].中国动物传染病学报,2018,26(3):45-50.
2022, Vol. 28 
冀公网安备13080202000677号