Abstract：Objective: To investigate the protective effect and mechanism of Palmatine on proliferation, apoptosis and cisplatin sensitivity in BRAF/KRAS mutant non-small cell lung cancer cells (NSCLC). Methods: The BRAF/KRAS mutant NSCLC cell line A549 was used as the research subject, and the effects of Palmatine on cell proliferation, apoptosis and cisplatin sensitivity were detected by thiazolyl blue method and flow cytometry, respectively. Western blotting was used to detect the expression of Bcl-2 and PARP protein. Results: Compared with the control group, the proliferation viability, Bcl-2 and PARP protein expression were significantly lower and the apoptosis rate was significantly higher in the 1 μmol/L, 5 μmol/L and 10 μmol/L groups (P<0.05). Compared with the control group, the proliferation viability, Bcl-2 and PARP protein expression were significantly reduced and the apoptosis rate was significantly increased in the palmatine and cisplatin groups (P<0.05); compared with the cisplatin group, the proliferation viability, Bcl-2 and PARP protein expression were further reduced and the apoptosis rate was further increased in the cisplatin+palmatine group (P<0.05). Conclusion: Palmatine can inhibit the proliferation of BRAF/KRAS mutant NSCLC cells, induce apoptosis, and enhance the sensitivity of cisplatin.
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2022, Vol. 28 
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