Abstract：Objective: To investigate the effects of ropivacaine on the inflammatory response and protein kinase A (PKA)/cAMP response element binding protein (CREB) pathway in rats with osteoarthritis (OA) pain. Methods: Rats were randomly divided into sham operation group, OA group (modeling) and low, medium, and high dose ropivacaine groups (modeling + intraperitoneal injection of 0.5, 1, 2 mg/kg ropivacaine), 16 in each group; the drug was administered after 2 weeks of modeling by cut off ligamenta cruciata, for two weeks. The tenderness threshold of rats was measured before model replication, 2 weeks after model replication, and 2 weeks after administration. Enzyme-linked immunoassay (ELISA) method was used to detect serum IL-1β, IL-6, and TNF-α levels, real-time fluorescent quantitative PCR method to determine the expression levels of IL-1β, IL-6, TNF-α, PKA, CREB mRNA in cartilage tissue, Western Blot to determine the levels of p-PKA/PKA and p-CREB/CREB in cartilage tissue, hematoxylin-eosin staining to observe cartilage tissue lesions.Results: After 2 weeks of administration, compared with the sham operation group, the tenderness threshold of rats in the OA group was significantly reduced (P<0.05), the serum IL-1β, IL-6, TNF-α levels, and cartilage tissue IL-1β, IL-6, TNF-α, PKA, CREB mRNA expression levels, p-PKA/PKA, p-CREB/CREB protein expression levels were significantly increased (P<0.05), the cartilage tissue had obvious pathological phenomena; compared with the OA group, the tenderness threshold of rats in the low, medium and high dose ropivacaine groups was significantly increased (P<0.05), the serum IL-1β, IL-6, TNF-α levels, and cartilage tissue IL-1β, IL-6, TNF-α, PKA, CREB mRNA expression levels, p-PKA/PKA, p-CREB/CREB protein expression levels were significantly reduced (P<0.05), and in a dose-dependent manner, the cartilage tissue lesions were alleviated.Conclusion: Ropivacaine can inhibit the activation of PKA/CREB pathway in rats with OA pain, reduce inflammation in the body, and reduce the degree of knee joint pain.
林玉美, 符明君, 陈基胜. 罗哌卡因对骨关节炎疼痛大鼠炎症反应及PKA/CREB通路的影响[J]. 河北医学, 2022, 28(5): 784-788.
LIN Yumei, FU Mingjun, CHEN Jisheng. The Effects of Ropivacaine on Inflammatory Response and PKA/CREB Pathway in Rats with Osteoarthritis Pain. HeBei Med, 2022, 28(5): 784-788.
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2022, Vol. 28 
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