Abstract：Objective: To investigate the effects of β-cryptoxanthin on receptor activator of NF-κB ligand (RANKL)/receptor activator of NF-κB (RANK)/osteoprotegerin (OPG) and osteoclast formation in orthodontic tooth movement model of rats. Methods: The rats were divided by random number table method into normal group, model group, β-cryptoxanthin low (3 μg), medium (6 μg) and high (12 μg) dose groups, with 10 rats in each group. Except for the normal group, all the other groups were established as orthodontic tooth movement models, and the administration of β-cryptocanthin was started on the first day after the installation of orthodontic appliances. 3μg, 6μg and 12μg of β-cryptocanthin were injected under the gingival mucosa of the left maxillary first molar near the middle of the rats in the low, medium and high dose groups respectively; the normal and model groups were injected with equal amounts of saline; the drug was administered once every 2 days for 28 days. The orthodontic tooth movement distance was measured 24h after the last dose in each group of rats. Periodontal membrane and alveolar bone tissues at the roots of maxillary first molars were taken from both sides and stained with hematoxylin-eosin (HE) and anti-tartrate acid phosphatase (TRAP), and the number of osteoclasts on the surface of alveolar bone on the pressure and tension sides were counted under light microscope. The expression levels of RANKL, RANK and OPG proteins in periodontal ligament and alveolar bone tissue at root were detected by Western blot. Results: Compared with the normal group, the periodontal space was narrowed on the pressure side and the periodontal ligament was widened on the tension side, osteogenic reactions were seen, TRAP staining was strongly positive on the pressure and tension sides, and TRAP staining was deeper on the pressure side than on the tension side. The levels of RANKL, RANK, OPG and RANKL/OPG ratio were all increased (P<0.05). Compared with the model group, the formation of new bone on the tension side of rats in the β-cryptoxanthin low, medium and high dose groups was gradually obvious, TRAP staining on the pressure side was strongly positive, TRAP staining on the tension side was weakly positive, and orthodontic tooth movement distance, the expression levels of RANKL and OPG protein in periodontal ligament and alveolar bone tissue at root were increased (P<0.05), and the number of osteoclasts on the surface of alveolar bone on the tension side, RANKL/ the number of osteoclasts and RANKL/OPG ratio on the alveolar bone surface on the tension side decreased (P<0.05). The changes of β-cryptoxanthin in each dose group were dose-dependent (P<0.05). Conclusions: β-cryptoxanthin may maintain the dynamic balance of osteogenesis and osteoclast resorption in periodontal ligament remodeling and alveolar bone reconstruction by up-regulating the expressions of RANKL and OPG, reducing the ratio of RANKL/OPG, blocking the combination of RANKL and RANK, promoting orthodontic tooth movement and inhibiting the further activation of osteoclasts.
王帅, 常颖, 席光伟, 沈娜. β-隐黄素对大鼠正畸牙移动模型RANKL RANK OPG通路及破骨细胞形成的影响[J]. 河北医学, 2022, 28(3): 362-367.
WANG Shuai, CHANG Ying, XI Guangwei, et al. Effects of β-Cryptoxanthin on RANKL/RANK/OPG Pathway and Osteoclast Formation in Orthodontic Tooth Movement Model of Rats. HeBei Med, 2022, 28(3): 362-367.
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2022, Vol. 28 
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