Abstract：Objective: To investigate the analgesic effect of ulinastatin (UTI) on rat model of neuropathic pain and its related molecular mechanism. Methods: Forty SD rats were randomly divided into sham group, CCI group, UTI group and UTI + Tacrolimus (FK506) group, with 10 rats in each group. Except sham group, chronic constriction injury (CCI) model was established in other groups, after the rats in each group were given the corresponding treatment, the changes of pain behavior were measured by pain behavior measurement, the morphological changes of sciatic nerve tissue were detected by hematoxylin eosin (HE) staining, and the levels of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor- α(TNF- α) in sciatic nerve tissue were detected by enzyme-linked immunosorbent assay (ELISA); TdT-mediated dUTP nick end labeling (TUNEL) was used to detect the apoptosis of rat sciatic nerve; Western blot was used to detect the protein expression of calcineurin (CN) and interleukin-10 (IL-10). Results: UTI can reduce CCI induced neuropathic pain in rats, alleviate the pathological injury of sciatic nerve tissue, and reduce the levels of IL-6, IL-1β and TNF-α in sciatic nerve tissue, and decrease the number of apoptosis of sciatic nerve cells, the expression of CN and IL-10 protein in sciatic nerve tissue of rats with neuropathic pain was significantly up-regulated, the difference was statistically significant (P<0.05). FK506, an inhibitor of CN, attenuated the effect of UTI on neuropathic pain rats, and down-regulated the protein expression of CN and IL-10 in sciatic nerve tissue of neuropathic pain rats, the difference was statistically significant (P<0.05). Conclusion: UTI may reduce inflammation and apoptosis in neuropathic pain rats by regulating CN/IL-10 signaling pathway, and then alleviate the neuropathic pain of rats.
黄钰媛, 韦雪梅, 郑育秀, 刘丽丽. 基于CN/IL-10信号途径探讨乌司他丁对神经性疼痛大鼠模型的镇痛效应及作用机制[J]. 河北医学, 2022, 28(2): 177-182.
HUANG Yuyuan, WEI Xuemei, ZHENG Yuxiu, et al. Analgesic Effect and Mechanism of Ulinastatin on Neuropathic Pain Rat Model Based on CN / IL-10 Signaling Pathway. HeBei Med, 2022, 28(2): 177-182.
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2022, Vol. 28 
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