Abstract：Objective: To observe the effect of Cyclopamine on cell proliferation and apoptosis in rats with benign prostatic hyperplasia. Methods: Thirty BPH rats were randomly divided into model group (BPH group) and Cyclopamine group (Cyclopamine group), and 15 rats without modeling served as normal control group (Normal group). Rats in Cyclopamine group were intraperitoneally injected with Cyclopamine, and rats in normal group and BPH group were intraperitoneally injected with the same dose of normal saline, once a day, for 7 consecutive days. After the administration, the rats were killed to obtain the prostate tissue of each group, and the indexes were determined. Results: Compared with normal group, the wet weight, volume and prostate index of BPH group and Cyclopamine group were significantly increased (P<0.05). The glandular epithelium showed papillary hyperplasia, and the number of glands increased, the glandular cavity enlarged, the stroma and blood vessels dilated with hyperemia and edema. The expression rate of apoptotic bodies was significantly decreased (P<0.05), and the proliferation rate was significantly increased (P<0.05). Compared with BPH group, the wet weight, volume and prostate index of Cyclopamine group were significantly lower [(3.18±0.28)g vs (2.03±0.22)g; (2.30±0.26)ml vs (1.45±0.27)ml; (0.78±0.23)% vs (0.46±0.17)%] (P<0.05). The histopathological changes of prostate were improved significantly. The expression rate of apoptotic bodies increased significantly [(3.17±0.68)% vs (5.25±0.77)%] (P<0.05). The proliferation rate of stromal and epithelial cells decreased significantly [OD 4d (1.35±0.09)/(1.62±0.17) vs (0.94±0.11)/(1.26±0.15); 5d (2.24±0.16)/(2.73±0.27) vs (1.74±0.17)/(2.16±0.20); cell number 4d (21.57±1.89)/(31.32±2.54) vs (17.88±1.26)/(25.44±2.39); 5d (33.66±3.16)/(44.97±4.29) vs (29.14±2.42)/(39.75±3.82)] (P<0.05). Conclusion: Cyclopamine can inhibit the proliferation of prostatic epithelial cells and stromal cells, and promote the occurrence of apoptosis.
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2022, Vol. 28 
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