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河北医学  2021, Vol. 27 Issue (10): 1603-1607    DOI: 10.3969/j.issn.1006-6233.2021.10.004
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NSUN2在口腔鳞癌中表达上调且通过NF-κB通路调控癌细胞发展
高朵朵, 王垚
延安大学附属医院, 陕西 延安 716000
Overexpression of NSUN2 Promoted Proliferation and Metastasis of Cervical Cancer Cells Through MiR-27b/FZD7 Axis
GAO Duoduo, WANG Yao
The Affiliated Hospital of Yan'an University, Shaanxi Yan'an 716000, China
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摘要 目的:探讨NSUN2对口腔鳞状细胞癌细胞增殖、侵袭和EMT的影响及其作用机制研究。方法:利用qRT-PCR检测NSUN2在口腔鳞状细胞癌患者组织、癌旁组织、口腔鳞状细胞癌OSCC细胞系(SCC9与CAL27细胞)和人正常口腔上皮角质细胞(HOK细胞)中的基因水平。将siRNA NC、NSUN2 siRNA1和NSUN2 siRNA2质粒分别转染CAL27细胞,分别利用MTT、细胞侵袭实验和Western blot检测CAL27细胞的增殖、侵袭和EMT能力。pcDNA-3.1(+)、pcDNA-NSUN2质粒转染CAL27细胞后,检测上调NSUN2对CAL27细胞的增殖、侵袭和EMT的影响。Western blot检测下调或过表达NSUN2对NF-κB通路相关蛋白表达的影响。NF-κB 通路抑制剂SN50作用细胞后,检测上调NSUN2对CAL27细胞发展的影响。结果:和癌旁组织相比,口腔鳞状细胞癌组织中NSUN2表达量明显上调(P<0.01);和HOK细胞相比,SCC9与CAL27细胞NSUN2表达量明显上调(P<0.01)。NSUN2 siRNA1和NSUN2 siRNA2转染组CAL27细胞增殖、侵袭和EMT能力明显低于siRNA NC组,p-TAK1/TAK1和NF-κB p-p65/NF-κB p65比值明显降低(P<0.01);pcDNA-NSUN2转染组CAL27细胞增殖、侵袭和EMT能力明显高于pcDNA-3.1(+)组,p-TAK1/TAK1和NF-κB p-p65/NF-κB p65比值明显上升(P<0.01)。SN50作用细胞后,NSUN2对CAL27细胞发展的促进作用明显下调。结论:NSUN2在口腔鳞状细胞癌细胞中表达上调且通过NF-κB通路促进CAL27细胞增殖、侵袭和EMT。
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高朵朵
王垚
关键词 NSUN2NF-κB口腔鳞状细胞癌增 殖    
AbstractObjective: To investigate the effect of NSUN2 on proliferation, invasion and EMT of oral squamous cell carcinoma and its mechanism. Methods: The gene levels of NSUN2 in the tissues of oral squamous cell carcinoma patients, paracancerous tissues, oral squamous cell carcinoma OSCC cell lines(SCC9 and CAL27 cells)and human normal oral epithelial keratinocytes(HOK cells)were detected by qRT-PCR. The siRNA NC, NSUN2 siRNA1 and NSUN2 siRNA2 plasmids were transfected into CAL27 cells, respectively. The proliferation, invasion and EMT ability of CAL27 cells were detected by MTT, cell invasion assay and Western blot, respectively. After transfected with pcDNA-3.1(+) and pcDNA-NSUN2 plasmid, the effects of up-regulation of NSUN2 on proliferation, invasion and EMT of CAL27 cells were detected. The effect of down-regulation or overexpression of NSUN2 on the expression of NF-κB pathway related proteins was detected by Western blot. NF-κB pathway inhibitor SN50 was used to detect the effect of up-regulation of NSUN2 on the development of CAL27 cells. Results: Compared with paracancerous tissues, the expression of NSUN2 in oral squamous cell carcinoma tissues was significantly up-regulated(P<0.01).Compared with HOK cells, NSUN2 expressions in SCC9 and CAL27 cells were significantly up-regulated(P<0.01).The proliferation, invasion and EMT abilities of CAL27 cells transfected with NSUN2 siRNA1 and NSUN2 siRNA2 were significantly lower than those of the siRNA NC group, and the ratio of p-TAK1/TAK1 and NF-κB p-p65/NF-κB p65 were significantly decreased(P<0.01);The proliferation, invasion and EMT abilities of CAL27 cells in pcDNA-NSUN2 transfected group were significantly higher than those in pcDNA-3.1(+) group, and the ratios of p-TAK1/TAK1 and NF-κB p-p65/NF-κB p65 were significantly increased(P<0.01). After treated with SN50, the promoting effect of NSUN2 on the development of CAL27 cells was significantly down-regulated. Conclusion: NSUN2 was up-regulated in oral squamous cell carcinoma cells and promoted the proliferation, invasion and EMT of CAL27 cells through NF-κB pathway.
Key wordsNSUN2    NF-κB    Oral squamous cell carcinoma    Proliferation
    
基金资助:陕西省重点研发计划项目,(编号:2019SF-228)
通讯作者: 王 垚   
引用本文:   
高朵朵, 王垚. NSUN2在口腔鳞癌中表达上调且通过NF-κB通路调控癌细胞发展[J]. 河北医学, 2021, 27(10): 1603-1607.
GAO Duoduo, WANG Yao. Overexpression of NSUN2 Promoted Proliferation and Metastasis of Cervical Cancer Cells Through MiR-27b/FZD7 Axis. HeBei Med, 2021, 27(10): 1603-1607.
链接本文:  
http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.10.004     或     http://www.hbyxzzs.cn/CN/Y2021/V27/I10/1603
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