三七总皂苷联合氨基胍治疗对糖尿病肾病大鼠肾功能 血CRP TNF-α MDA和SOD水平的影响

doi:10.3969/j.issn.1006-6233.2021.09.002

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1610 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要 目的: 探究三七总皂苷(PNS)联合氨基胍(AG)治疗对糖尿病肾病大鼠肾功能、血C-反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平的影响。方法: 取清洁级雄性SD大鼠75只,随机分为正常对照组(15只)和实验组(60只),实验组腹腔注射链脲佐菌素建立糖尿病肾病大鼠模型,平衡喂养3d,将建模成功的实验组大鼠随机分为模型组(13只)、PNS组(13只)、AG组(13只)、联合组(13只),予以对应药物灌胃4周。观察各组大鼠肾脏组织病理形态变化,并比较各组大鼠肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α、血清MDA、SOD水平。结果: 正常对照组肾小球外观规则完整,系膜细胞稀少,基底膜薄;肾小管形状清晰,基底膜无改变,实验组肾小球明显增大,系膜细胞增加,基底膜增厚,肾小管无明显改变;相比模型组,PNS组、AG组、联合组肾小球病理状态明显改善。模型组、PNS组、AG组、联合组肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α水平高于正常对照组,PNS组、AG组、联合组肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α水平低于模型组,联合组肾小球容量、血糖、血肌酐、24h尿蛋白、血浆CRP、TNF-α水平高于PNS组、AG组:差异均有统计学意义(P<0.05)。模型组、PNS组、AG组、联合组MDA水平高于正常对照组,SOD水平低于正常对照组;PNS组、AG组、联合组MDA水平低于模型组,SOD水平高于模型组;联合组SOD水平高于AG组:差异均有统计学意义(P<0.05)。结论: PNS联合AG治疗对糖尿病肾病可显著改善肾小球病理状态和肾小球功能,降低血浆炎症因子CRP、TNF-α水平,减轻氧化应激水平。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	郑杰洪
	郑映苗
	陈凤梧

关键词 ：糖尿病肾病, 大鼠, 肾功能, 氧化应激, 三七总皂苷, 氨基胍

Abstract：Objective: To explore the effects of panax notoginseng saponins combined with aminoguanidine treatment on renal function, blood C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA) and superoxide dismutase in diabetic nephropathy rats ( SOD) level. Methods: 75 clean-grade male SD rats were randomly divided into normal control group (15 rats) and experimental group (60 rats). The experimental group was intraperitoneally injected with streptozotocin to establish a rat model of diabetic nephropathy. The rats in the experimental group were randomly divided into model group (13 rats), PNS group (13 rats), AG group (13 rats), and combination group (13 rats). The rats were given corresponding drugs by gavage for 4 weeks. Observe the pathological changes of rat kidney tissues in each group, and compare the levels of glomerular volume, blood glucose, blood creatinine, 24h urine protein, plasma CRP, TNF-α, serum MDA and SOD in each group. Results: The glomerulus in the normal control group had a regular and complete appearance, with few mesangial cells and thin basement membrane; the shape of the renal tubules was clear, and the basement membrane was unchanged. The glomerulus of the experimental group was significantly enlarged, mesangial cells increase, basement membrane thickens, and renal tubules. There was no significant change; compared with the model group, the glomerular pathology of the PNS group, AG group, and combination group was significantly improved. The glomerular volume, blood glucose, blood creatinine, 24h urine protein, plasma CRP, and TNF-α level of the model group, PNS group, AG group, and combination group were higher than those of the normal control group, PNS group, AG group, and combination group. Blood glucose, blood creatinine, 24h urine protein, plasma CRP, and TNF-α level were lower than the model group. The combined group had higher glomerular volume, blood glucose, blood creatinine, 24h urine protein, plasma CRP, and TNF-α level than the PNS group. AG group: The differences were statistically significant (P<0.05). The MDA level of the model group, PNS group, AG group, and the combination group was higher than the normal control group, and the SOD level was lower than the normal control group; the MDA level of the PNS group, the AG group, and the combination group was lower than the model group, and the SOD level was higher than the model group; The SOD level of the group was higher than that of the AG group: the differences were statistically significant (P<0.05). Conclusion: PNS combined with AG treatment can significantly improve glomerular pathology and glomerular function in diabetic nephropathy, reduce plasma inflammatory factors CRP and TNF-α level, and reduce oxidative stress level.

Key words： Diabetic nephropathy Rats Renal function Oxidative stress Panax notoginseng saponins Aminoguanidine

基金资助:广东省汕头市科技计划医疗卫生项目,(编号:190531125267683 )

引用本文:

郑杰洪, 郑映苗, 陈凤梧. 三七总皂苷联合氨基胍治疗对糖尿病肾病大鼠肾功能血CRP TNF-α MDA和SOD水平的影响[J]. 河北医学, 2021, 27(9): 1414-1417.
ZHENG Jiehong, ZHENG Yingmiao, CHEN Fengwu. Effects of Panax Notoginseng Saponins Combined with Aminoguanidine Treatment on Renal Function Blood CRP TNF-α MDA and SOD Levels in Diabetic Nephropathy Rats. HeBei Med, 2021, 27(9): 1414-1417.

链接本文:

http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.09.002 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I9/1414

[1] Sakin A,Sahin S,Behlul A,et al.The association of visfatin levels with metabolic parameters and inflammation in diabetic nephropathy[J].Eastern Journal of Medicine,2020,25(2):218~224.
[2] 郭丽芳,孟皓波,高爱华,等.不同分期的糖尿病肾病病人炎性因子及氧化应激指标的变化及意义研究[J].内蒙古医学院学报,2019,41(4):370~373.
[3] 唐标,唐文静,戴姿薇,等.三七总皂苷抑制缺氧缺糖/复氧复糖诱导的SH-SY5Y细胞焦亡[J].中国病理生理杂志,2020,36(7):1178~1184.
[4] 方洁,邓雅欣,王鹏宇,陈曦,邵德翠,汪萌芽.氨基胍通过减轻内质网应激改善糖尿病小鼠认知功能[J].皖南医学院学报,2019,38(5):409~413.
[5] 薛耀明.重视糖尿病肾脏病患者的心血管风险评估与管理[J].中华糖尿病杂志,2020,12(10):761~764.
[6] 李洁超,胡亚芬,吴震宇,等.糖尿病肾病与原发性肾病综合征患者血脂,血压,血清白蛋白的对比[J].中国医师杂志,2020,22(7):1096~1098.
[7] Raval N,Kumawat A,Kalyane D,et al.Understanding molecular upsets in diabetic nephropathy to identify novel targets and treatment opportunities[J].Drug Discovery Today,2020,25(5):862~878.
[8] 傅奕,陈帮明,李鑫,等.益肾化湿颗粒联合雷公藤多苷片治疗糖尿病肾病的疗效研究[J].中草药,2020,512(23):130~134.
[9] 穆亚敏,宋志勇.氨基胍治疗大鼠肺纤维化的效果及相关机制研究[J].基因组学与应用生物学,2019,38(3):1335~1340.
[10] 侯凯健,郑杰洪,朱丹,等.三七总皂苷联合氨基胍治疗对糖尿病肾病大鼠的肾脏保护作用及机制[J].中华中医药学刊,2020,38(8):237~242.
[11] 崔翠.前列地尔联合雷公藤多苷治疗糖尿病肾病的疗效观察及对炎性因子的影响[J].中国基层医药,2019,26(15):1866~1870.
[12] 高欣彤,杨慧慧.肾衰宁颗粒联合贝那普利治疗糖尿病肾病对患者血糖及氧化应激指标的影响[J].海南医学,2020,31(23):53~56.