Abstract：Objective: To investigate the effect of Danbai Granule on endometrial fibrosis in rats with intrauterine adhesion (IUA) and its possible mechanism. Methods: SD rats were randomly divided into sham operation group (15 rats) and model group (65 rats), the IUA model was established by mechanical curettage and infective injury, and the sham operation only underwent laparotomy and uterine suture. The successful models were divided into model group, Danbai Granule low-dose group (2.5 g/kg), Danbai Granule middle-dose group (5.0 g/kg) and Danbai Granule high-dose group (10.0 g/kg), with 15 in each group. The drug was administered 24 hours after successful modeling, Danbai Granule low, medium and high dose groups was given by gavage according to the dosage, while the sham operation group and the model group were given the same amount of normal saline, the time of intragastric administration was from 7∶00 to 10∶00 a.m, continuous gavage for 24 days. At 24 hours after the last administration, the uterine tissues were collected, the uterine permeability and AFS score were measured, hematoxylin eosin staining (HE) was used to detect the pathological changes, endometrial thickness and the number of glands in each group. Masson staining was used to detect the degree of endometrial fibrosis; enzyme linked immunosorbent assay (ELISA) was used to detect the collagen content in rat uterus; and Western blot was used to detect the expression of transforming growth factor-β1 (TGF-β1)/Smad pathway protein in endometrium. Results: Compared with those in the sham operation group, the uterine cavity disappeared; the cavity wall tissue showed adhesion state, the endometrial thickness and the number of glands were significantly reduced in the model group; the uterine patency score, AFS score, content of insoluble collagen and area of endometrial fibrosis were significantly higher; the protein expression levels of TGF-β1 and Smad3 in endometrium were significantly higher, and the expression level of Smad7 protein were significantly lower (P<0.05); compared with those in the model group, the endometrial thickness and the number of glands in the Danbai Granule low, medium and high dose groups were higher to a certain extent, the patency of uterus and the degree of endometrial fibrosis were relieved, and the content of insoluble collagen was significantly lower; the protein expression levels of TGF-β1 and Smad3 showed an decreasing trend; the expression of Smad7 protein showed an increasing trend (P<0.05); there was a certain dose effect relationship. Conclusion: Danbai granule can effectively alleviate the degree of endometrial fibrosis in IUA rats, which may be related to the inhibition of TGF-β/Smad pathway.
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2021, Vol. 27 
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