黄芪注射液联合阿托伐他汀对急性肺损伤大鼠肺纤维化及细胞黏附功能的影响

doi:10.3969/j.issn.1006-6233.2021.05.04

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摘要 目的: 探讨黄芪注射液联合阿托伐他汀对急性肺损伤大鼠肺纤维化及细胞黏附功能的影响。方法: Wistar大鼠60只,随机分为假手术组(对照组)、模型组、阿托伐他汀组(他汀组)、黄芪组及黄芪联合阿托伐他汀组(联合组),每组12只。建立急性肺损伤肺纤维化模型,实验开始后黄芪组和联合组每天腹腔注射给予1.0mL·kg^-1·d^-1的黄芪注射液,他汀组和联合组给予阿托伐他汀20mgl·kg^-1·d^-1灌胃;同时对照组、模型组和他汀组给予腹腔注射等量生理盐水;对照组、模型组和黄芪组给予等量生理盐水灌胃。各组于第14天给药6h后处死大鼠,做组织病理学观察和评分,并检测各组肺组织羟脯氨酸(HyP)、肿瘤坏死因子α(TNF-α)、细胞间黏附分子(ICAM-1)、血管细胞间黏附分子(VCAM-1)及血小板内皮细胞黏附分子(PECAM-1)的表达变化。结果: 与对照组相比:其余四组HyP、TNF-α、ICAM-1、VCAM-1及PECAM-1表达以及肺泡炎、肺纤维化评分均显著增强(P<0.05);与模型组比较:他汀组、黄芪组及联合组肺组织HyP、TNF-α、ICAM-1、VCAM-1及PECAM-1表达明显较低(P<0.05),且联合组更为显著。结论: 急性肺损伤肺纤维化组织中ICAM-1、VCAM-1、PECAM-1、TNF-α及HyP的表达显著升高,而黄芪、他汀组和联合组均能明显调节其失衡状态,降低肺泡炎和肺纤维化评分,从而能有效减缓急性肺损伤肺纤维化的发生和发展,且联合组效果更明显。

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关键词 ：急性肺损伤肺纤维化, 黄芪, 阿托伐他汀, 黏附分子, 肿瘤坏死因子α, 羟脯氨酸

Abstract：Objective: To study the effects of astragalus injection combined with atorvastatin on pulmonary fibrosis and cell adhesion function in rats with acute lung injury.Methods: 60 wistar rats were randomly divided into sham operation group (control group),model group,atorvastatin group (statin group),Astragalus group and astragalus group combined atorvastatin group (combination group),with 12 rats in each group.The pulmonary fibrosis model of acute lung injury was established.After the beginning of the experiment,the Astragalus group and the combined group were intraperitoneally injected with 1.0ml·kg^-1·d^-1 astragalus injection every day,and the statin group and the combined group were given atorvastatin 20 mL ·kg^-1·d^-1 gavage.At the same time,the control group,model group and statin group were given intraperitoneal injection of the same amount of normal saline.The control group,model group and Astragalus group were given the same amount of normal saline.The rats in each group were sacrificed 6h after administration on day 14 for histopathological observation and scoring,and the expression changes of Hydroxyproline (HyP),tumor necrosis factor (TNF-α),intercellular adhesion molecule (ICAM-1),vascular intercellular adhesion molecule (VCAM-1) and platelet endothelial adhesion molecule (PECAM-1) in lung tissues of each group were detected.Results: Compared with the control group,HyP,TNF-α,ICAM-1,VCAM-1 and PECAM-1 expression,alveolitis and pulmonary fibrosis scores of the other four groups were significantly enhanced (P<0.05).Compared with the model group,HyP,TNF-α,ICAM-1,VCAM-1 and PECAM-1 in the lung tissues of the statins group,the Astragalus group and the combination group were significantly lower (P<0.05),and the roots of the combination group were significant.Conclusion: The expressions of ICAM-1,VCAM-1,TNF-α,PECAM-1 and HyP in the pulmonary fibrosis tissues with acute lung injury were significantly unbalanced,while the astragalus,statins and the combined group were able to significantly correct their imbalances,reduce alveolitis and pulmonary fibrosis scores,and thus effectively slow down the occurrence and development of pulmonary fibrosis with acute lung injury.Moreover,the combined group was more effective..

Key words： Acute lung injury pulmonary fibrosis Astragalus Atorvastatin Adhesive molecule TNF-α Hydroxyproline

基金资助:湖北省卫生计生委医药院校专项科研课题,(编号:WJ2016-Y-41);湖北省黄冈市科技计划项目,(编号:20150205)

通讯作者: 李火平

引用本文:

周易, 李火平, 夏光明, 周帆, 金朝霞, 张慧. 黄芪注射液联合阿托伐他汀对急性肺损伤大鼠肺纤维化及细胞黏附功能的影响[J]. 河北医学, 2021, 27(5): 719-723.
ZHOU Yi, LI Huoping, XIA Guangming, et al. Effects of Astragalus Injection Combined with Atorvastatin on Pulmonary Fibrosis and Leukocyte Adhesion Function in Rats with Acute Lung Injury. HeBei Med, 2021, 27(5): 719-723.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.05.04 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I5/719

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