Abstract：Objective: To investigate the effect of ovariectomy on aortic lipid metabolism in female hyperlipidemia rats. Methods: Healthy female SD rats were randomly divided into blank group,model group 1 ; model group 2,hyperlipidemia rat model was established in model group 1,and ovariectomized+ hyperlipidemia rat model was established in model group 2. After 8 weeks,the mRNA levels of CAV1,LDLR,VLDLR,ABCA1,ApoA1 and SRB1 were detected by real-time quantitative PCR,serum total cholesterol,triglyceride,low density lipoprotein cholesterol and high density lipoprotein cholesterol of rats were determined by Elisa. Results: Compared to the blank group,the TC、TG、LDL-C contents of model 1 and model 2 increased significantly (P<0.01),the HDL-C content decreased significantly (P<0.01),the TC,TG,LDL-C content of model 2 group was significantly higher than that of model 1 group (P<0.01),The HDL-C content was significantly less than that of model 1 group (P<0.01).Compared with the blank group,the mRNA relative expression of ABCA1,ApoA1,CAV1,SRB1,VLDLR,LDLR in model group 1 and model group 2 decreased significantly (P<0.01),and the expression in model group 2 was lower than that in model group 1(P<0.01). Conclusion: Ovariectomy leads to the decrease of estrogen and affects aortic lipid metabolism in female rats by ABCA1,ApoA1,CAV1,SRB1,VLDLR,LDLR mRNA,resulting in abnormal serum lipid content in rats.
[1] Di Y,Hu CJ,Deng XY,et al.Therapeutic effect of chitooligosaccharide tablets on lipids in high-fat diets induced hyperlipidemic rats[J]. Molecules,2019,24: 1~16. [2] Shrivastava A,Chaturvedi U,Singh SV,et al.Lipid lowering and antioxidant effect of miglitol in triton treated hyperlipidemic and high fat diet induced obese rats[J]. Lipids,2013,48(6): 597~607. [3] 王健章,俞赟丰,周曼丽,等.冠心病血瘀证形成过程动物模型的心电图变化[J].中医药信息,2020,3(1): 1~5. [4] Han HJ,Song XJ,Yadav D,et al.Ulmus macrocarpa Hance modulates lipid metabolism in hyperlipidemia via activation of AMPK pathway[J]. PLoS One,2019,14(5): 1~14. [5] S BA,Pedroza-Tobias C,Medina L,et al. Global overview of the epidemiology of atherosclerotic cardiovascular disease[J]. Arch Med Res,2015,46(5): 328~338. [6] Brown AJ,Teng Z,Evans PC,et al.Role of biomechanical forces in the natural history of coronary atherosclerosis[J]. Nature Reviews,2016,13(4):210~220. [7] Sakamuri SS,Higashi Y,Sukhanov S,et al.TRAF3IP2 mediates atherosclerotic plaque development and vulnerability in ApoE mice[J]. Atherosclerosis,2016,252:153~160. [8] Zhang Y,Ma KL,Ruan XZ,et al.Dysregulation of the low-density lipoprotein receptor pathway is involved in lipid disorder-mediated organ injury[J]. International Journal of Biological Sciences,2016,12(5): 569~579.
2021, Vol. 27 
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