穿龙薯蓣皂苷元对CIA小鼠脾脏Treg细胞的影响

doi:10.3969/j.issn.1006-6233.2021.01.004

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摘要 目的: 在胶原性关节炎小鼠模型中初步探讨穿龙薯蓣皂苷元对其脾脏Treg细胞的影响。方法: 通过将鸡Ⅱ型胶原乳剂与弗氏完全佐剂混合后免疫DBA1/J小鼠,建立胶原性关节炎(CIA)模型。通过免疫磁珠分离法(MACS)分选CIA小鼠脾脏中的CD4⁺CD25⁺Treg细胞。流式细胞术检测Treg细胞的纯度,并体外培养。穿龙薯蓣皂苷元作用48h后,流式细胞术检测Treg细胞比例,实时荧光定量PCR法检测其转录因子Foxp3的表达,ELISA法检测相关细胞因子IL-10的水平。结果: 成功建立CIA模型小鼠。MACS法分选CIA小鼠脾脏Treg细胞纯度达90%以上。穿龙薯蓣皂苷元作用48h后,与对照组相比,给药组Treg细胞比例显著升高(P<0.05),Foxp3　mRNA表达显著升高(P<0.05),细胞上清中IL-10的水平显著升高(P<0.05)。结论: 穿龙薯蓣皂苷元能显著提高CIA小鼠脾脏中Treg细胞的比例、转录因子Foxp3和相关细胞因子IL-10的水平。

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关键词 ： CIA模型, 调节性T细胞, Foxp3, 白细胞介素-10, 薯蓣皂苷元

Abstract：Objective: To evaluate the effect of diosgenin on regulatory Treg cells in spleen of collagen induced arthritis (CIA) mice. Methods: The CIA mice model was established by immunizing DBA1/J mice with chicken type II collagen and Freund’s completed adjuvant. And CD4⁺CD25⁺Treg cells in spleen of CIA model mice were isolated by MACS. Then, to detect the purity of Treg cells by flow cytometry and culture them in vitro. 48 hours after treatment, the proportion of Treg cells was detected by flow cytometry, the expression of transcription factor Foxp3 in Treg cells was detected by real-time fluorescence quantitative PCR, and the levels of IL-10 were detected by ELISA. Results: CIA model mice were established successfully. The purity of Treg cells isolated by MACS was more than 90%. Compared with the control group, the percentage of Treg cells, the Foxp3 mRNA level, and the expression of IL-10 in cell supernatant were increased significantly (P<0.05) 48 hours after treatment.Conclusion: Diosgenin can significantly increase the proportion of Treg cells, the levels of transcription factor Foxp3 and IL-10 in CIA mice.

Key words： CIA model Regulatory T cells Foxp3 Interleukin-10 Diosgenin

基金资助:国家自然科学基金项目,(编号:81273986);河北省中医药管理局科研计划项目,(编号:2015155);河北省高等学校科学技术研究项目,(编号:QN2016031)

通讯作者: 宋鸿儒

引用本文:

梁秀军, 代俊利, 李强, 郭亚春, 高亚贤, 宋鸿儒. 穿龙薯蓣皂苷元对CIA小鼠脾脏Treg细胞的影响[J]. 河北医学, 2021, 27(1): 15-17.
LIANG Xiujun, et al. Effects of Diosgenin on Treg Cell in Spleen of CIA Mice. HeBei Med, 2021, 27(1): 15-17.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.01.004 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I1/15

[1] Bakheet SA,Ansari MA, Nadeem A,et al. CXCR3 antagonist AMG487 suppresses rheumatoid arthritis pathogenesis and progression by shifting the Th17/Treg cell balance[J]. Cellular Signalling, 2019, 64: 109395.
[2] Wang C, Mu Z. Research progress on regulatory T cells and their relationship with autoimmune diseases[J]. Science Journal of Public Health, 2020, 8(3):83.
[3] 伊刚,赵毅,解丰,等.单细胞RNA测序揭露人FOXP3+调节性T细胞稳定性的关键调控分子(英文)[J].Science Bulletin,2020,65(13):1114~1124.
[4] 郭亚春,梁秀军,高亚贤,等.中药薯蓣皂苷元对CIA大鼠滑膜组织VEGF及AP-Ⅰ表达的影响[J].世界科学技术-中医药现代化,2015,17(9):1801~1805.
[5] 高亚贤,王永为,宋鸿儒,等.穿龙薯蓣皂苷元体外对CIA小鼠Treg细胞亚群及转录因子的影响[J].中国免疫学杂志,2019,35(21):2593~2598.
[6] 李智伟,田永芳,卢佩佩,等.类风湿性关节炎患者淋巴细胞和Treg/Th17平衡的检测及意义[J].国际检验医学杂志,2019,40(16):2026~2028.
[7] Liebling MR. Methylation of the CTLA-4 promoter and treg cell dysfunction in rheumatoid arthritis: comment on the article by cribbs et al.Treg cell function in rheumatoid arthritis is compromised by CTLA-4 promoter methylation resulting in failure to activate the indoleamine 2,3-dioxygenase pathway. Arthritis Rheumatol 2014;66[J]. Arthritis & rheumatology (Hoboken, N.J.), 2015, 67(5): 1406.
[8] Zavvar M,Assadiasl S,Zargaran S, et al. Adoptive Treg cell-based immunotherapy: Frontier therapeutic aspects in rheumatoid arthritis[J]. Immunotherapy, 2020, 12(12): 933~946.
[9] 郭亚春,赵晓菲,黄群,等.薯蓣皂苷元体外作用对胶原诱导性关节炎小鼠Th1/Th2型细胞因子的影响[J].中国老年学杂志,2018,38(13):3193~3196.
[10] Liang XiuJun, Guo Yachun, Sun Tongyou, et al. Anti-angiogenic effect of total saponins of rhizoma dioscorea nipponica on collagen induced-arthritis in rats[J]. Experimental and therapeutic medicine, 2016, 12(4): 2155~2160.