Abstract：Objective: To investigate the effects of isoflurane on the formation of collateral circulation and Notch pathway in rats with cerebral ischemia-reperfusion. Methods: 90 clean grade SD rats were random divided into control group, model group, edaravone group (3 mg/kg), isoflurane low-dose group (50 μg/kg), isoflurane high-dose group (100 μg/kg), 18 in each group. The rats in the model group, edaravone group, isoflurane low-dose group and high-dose group were established cerebral ischemia-reperfusion model. After successful modeling, the rats in the edaravone group, isoflurane low-dose group and high-dose group on the first day after operation were intraperitoneally administrated corresponding drugs, once a day, for 1 week. The rats in the control group and the model group were given the same volume of saline. After the experiment, the microvessel density and neuromotor function scores in the reimplantation area of the rat brain were measured, and the results of the bilateral sticker removal and balance beam crossing experiment were obtained. The neuron structure in the reimplantation area of the rat brain injury was observed. And real-time fluorescence quantitative reversal Record-polymerase chain reaction (RT-PCR) and Western-blot (Western-blot) method to measure Notch1, vascular metabolic growth factor (VEGF) mRNA and protein levels. Results: The neuron cells in the control group were intact; a large number of necrotic neurons were seen in the hippocampus of the model group, the nerve cell nucleus was contracted, and there was significant inflammatory cell infiltration; the neuronal cell structure in the edaravone group and the isoflurane high-dose group was relatively complete, and the inflammatory cell infiltration Reduced; significant infiltration of inflammatory cells is still visible in the low-dose isoflurane group. Compared with the control group, the microvascular density in cerebral ischemia-reperfusion area, Notch1, VEGF mRNA and protein levels in the model group were decreased, and the neuromotor function score, the removal time of bilateral stickers and the time of passing the balance beam increased (P<0.05). Compared with the model group, microvascular density in cerebral ischemia-reperfusion area, Notch1, VEGF mRNA and protein levels in the edaravone group, the low-dose isoflurane group and the high-dose isoflurane group were increased, and the neuromotor function score, the removal time of bilateral stickers and the time of passing the balance beamdecreased (P<0.05). The microvascular density in cerebral ischemia-reperfusion area, Notch1, VEGF mRNA and protein levels of high dose isoflurane group were higher than those of low dose isoflurane group, and the neuromotor function score, the removal time of bilateral stickers and the time of passing the balance beam lower than those of low dose isoflurane group (P<0.05). There were no significant changes in microvascular density in cerebral ischemia-reperfusion area, Notch1, VEGF mRNA and protein levels, neuromotor function score, bilateral Decal removal time and balance beam passing time of rats between the edaravone group and high dose isoflurane group (P>0.05). Conclusion: Isoflurane can promote the formation of collateral circulation in rats with cerebral ischemia-reperfusion, and its mechanism may be related to that isoflurane can promote the expression of Notch1, VEGF mRNA and protein in rats with cerebral ischemia-reperfusion and then activate Notch signaling pathway.
张敏, 项明琼, 油文静, 伍璀. 异氟醚对脑缺血再灌注大鼠侧支循环形成及Notch通路的影响[J]. 河北医学, 2021, 27(1): 5-10.
ZHANG Min, XIANG Mingqiong, YOU Wenjing, et al. Effects of Isoflurane on the Formation of Collateral Circulation and Notch Pathway in Rats with Cerebral Ischemia-reperfusion. HeBei Med, 2021, 27(1): 5-10.
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2021, Vol. 27 
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