Abstract：Objective: To investigate the protective effects and mechanism of Ganoderma lucidum polysaccharides(GLPS)on doxorubicin (DOX)-induced injury of H9c2 cells.Methods: H9c2 cells were divided into control group,GLPs high dose group,DOX group,GLPs low and high dose + DOX group.Cell viability was detected by AV-PI method,and apoptosis related protein expression was detected by Western blot.Results: The apoptosis rate and the expression of apoptosis related proteins in the DOX group were significantly higher than those in the control group,but the apoptosis rate and the expression of Caspase-3,Caspase-8,cleared PARP and Bax in the GLPs low and high dose + DOX group were significantly lower than those in the DOX group,and the expression of Bcl-2 was significantly higher,and the Ganoderma lucidum polysaccharide was dose-dependent.Conclusion: Ganoderma lucidum polysaccharide can reduce the toxicity of doxorubicin induced cardiomyocytes,and its mechanism may be related to its inhibition of cardiomyocyte apoptosis.
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2020, Vol. 26 
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