环磷酰胺对百草枯中毒家兔肺过氧化损伤的干预作用

doi:10.3969/j.issn.1006-6233.2020.11.08

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摘要 目的: 探讨环磷酰胺对百草枯中毒家兔肺过氧化损伤的干预作用。方法: 将40例健康家兔随机分为对照组、模型组、甲强龙组、环磷酰胺+甲强龙组,每组10例。模型组、甲强龙组、环磷酰胺+甲强龙组均给予百草枯灌胃染毒,甲强龙组染毒后给予甲强龙治疗,环磷酰胺+甲强龙组给予环磷酰胺和甲强龙,对照组和模型组腹腔注入生理盐水。采集各组动物治疗1d、3d、5d、7d时耳缘静脉血,检测血清TGF-β₁、MDA及SOD水平。结果: 治疗1d后,模型组、甲强龙组、环磷酰胺+甲强龙组血清TGF-β₁、MDA水平均高于对照组,血清SOD水平则低于对照组(P<0.05)。治疗3d、5d、7d后,甲强龙组、环磷酰胺+甲强龙组血清TGF-β₁、MDA水平均低于模型组,血清SOD水平则高于模型组(P<0.05)。环磷酰胺+甲强龙组在治疗3d、5d、7d后血清TGF-β₁、MDA水平均低于甲强龙组,血清SOD水平则高于甲强龙组(P<0.05)。结论: 环磷酰胺能有效降低血清TGF-β₁、MDA的表达和提高SOD活性,从而改善百草枯中毒引起的过氧化损伤。

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关键词 ：环磷酰胺, 百草枯, 过氧化损伤, 转化生长因子-β₁

Abstract：Objective: To investigate the effect of cyclophosphamide on lung peroxidation injury of rabbits with paraquat poisoning.Methods: 40 healthy rabbits were randomly divided into control group,model group,methylpolygonon group and cyclophosphamide+methylpolygonon group,with 10 cases in each group.Paraquat was given to the model group,methylpolygonon group and cyclophosphamide + methylpolygonon group.The methylprednisolone group was treated with methylprednisolone,the cyclophosphamide + methylprednisolone group was treated with cyclophosphamide and methylprednisolone,and the control group and model group were given normal saline.Results: After 1 day of treatment,the levels of TGF - β 1 and MDA in model group,methylprednisolone group and cyclophosphamide + methylprednisolone group were higher than those of control group,while serum SOD level was lower than that of control group (P<0.05).After 3 days,5 days and 7 days of treatment,the levels of serum TGF - β 1 and MDA in methylprednisolone group and cyclophosphamide + methylprednisolone group were lower than those of model group,while serum SOD level was higher than that of model group (P<0.05).The levels of serum TGF - β 1 and MDA in the cyclophosphamide + methylprednisolone group were lower than those in the methylprednisolone group after 3 days,5 days and 7 days of treatment,while the serum SOD level was higher than that of the methylprednisolone group (P<0.05).Conclusion: Cyclophosphamide can effectively reduce the expression of serum TGF-β₁ and MDA and increase the activity of SOD,so as to improve the peroxidation damage caused by paraquat.

Key words： Cyclophosphamide Paraquat Peroxide damage Transforming growth factor-β₁

基金资助:重庆市第三批区域医学重点学科建设项目,(编号:zdxk201811);重庆市涪陵区科技计划项目,(编号:FLKJ2018BBB3025)

通讯作者: 简万均

引用本文:

蒋昌华, 简万均, 符宜龙, 唐中建, 舒艾娅. 环磷酰胺对百草枯中毒家兔肺过氧化损伤的干预作用[J]. 河北医学, 2020, 26(11): 1792-1796.
JIANG Changhua, JIAN Wanjun, FU Yilong, et al. Intervention Effect of Cyclophosphamide on Lung Peroxidation Injury Induced by Paraquat in Rabbits. HeBei Med, 2020, 26(11): 1792-1796.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2020.11.08 或 http://www.hbyxzzs.cn/CN/Y2020/V26/I11/1792

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