Abstract：Objective: To study the expression and significance of microRNA-125b (mir-125b) and placental growth factor (PIGF) in villi and serum of women with early abortion.Method: 105 cases of early spontaneous aborted women admitted to our hospital from July 2017 to October 2019 were selected as the research objects,and 105 cases of induced abortion in the same period were selected as the control group.Peripheral venous blood serum was collected and chorionic tissue was separated from all abortion tissues.Real-time quantitative PCR was used to detect the mRNA expression levels of miR-125b and PIGF in serum and villus tissue,and immunoblotting (WB) was used to detect the protein expression levels of PIGF in villus tissue.The serum PIGF level was detected by enzyme linked immunosorbent assay (ELISA),and the correlation of mir-125b and PIGF protein levels in serum and chorionic tissue was analyzed by Pearson method.The predictive value of serum mir-125b and PIGF levels in patients of spontaneous abortion in early pregnancy was analyzed by receiver operating characteristic (ROC).Result: Compared with the control group,the level of mir-125b in chorionic tissue of pregnant women in spontaneous abortion group increased significantly,and the level of PIGF mRNA and protein decreased significantly (P<0.05);The level of miR-125b in serum increased significantly and the level of PIGF decreased significantly;The difference was statistically significant (P<0.05).Pearson analysis showed that there was a negative correlation between mir-125b level and PIGF protein level in chorionic tissue,serum of spontaneous abortion pregnant women (r=-0.643,P<0.05).Serum levels of mir-125b and PIGF were 0.896 (95% CI:0.850-0.943) and 0.884 (95% CI:0.838-0.929) respectively for predicting the area under curve (AUC) of spontaneous abortion in early pregnancy.The sensitivity and specificity of mir-125b (>1.630) in predicting spontaneous abortion were 95.30% and 84.60%,respectively.The sensitivity and specificity of PIGF (<42.553 pg/mL) in predicting spontaneous abortion were 90.60% and 77.90%;the AUC,specificity and sensitivity of combined detection for predicting spontaneous abortion in early pregnancy were 0.954,92.30% and 89.60%,respectively.Conclusion: The levels of mir-125b and PIGF in chorionic tissues of women suffering early spontaneous abortion are up-regulated,and they are negatively correlated,which may have some predictive value for the occurrence of spontaneous abortion.
杨艳华, 吴军, 严格. Mir-125b和PIGF在早期流产孕妇绒毛组织及血清中的表达及意义[J]. 河北医学, 2020, 26(11): 1780-1785.
YANG Yanhua, WU Jun, YAN Ge. Expression of Mir-125b and PIGF in Villus Tissue and Serum of Women Suffering Early Abortion and Its Significance. HeBei Med, 2020, 26(11): 1780-1785.
[1] Zhang X,Wang Y,Zhao N,et al.Variations in chromosomal aneuploidy rates in IVF blastocysts and early spontaneous abortion chorionic villi[J].Assist Reprod Gen,2020,37(4):527~537. [2] 吴成平,张婵.复发性流产患者胎盘绒毛组织中MMP2、MMP9、VEGF的表达水平[J].热带医学杂志,2018,18(5):598~602. [3] Gu Y,Zhao S,Wan J,et al.Hsa-miRNA-125b may induce apoptosis of HTR8/SVneo cells by targeting MCL1[J].Reprod Biol,2019,19(4):368~373. [4] Salvolini E,Vignini A,Sabbatinelli J,et al.Nitric oxide synthase and VEGF expression in full-term placentas of obese women[J].Histochem Cell Biol,2019,152(6):415~422. [5] Soukup V,Capoun O,Pesl M,et al.Placental growth factor in bladder cancer compared to the diagnostic accuracy and prognostic performance of vascular endothelial growth factor A[J].Anticancer Res,2018,38(1):239~246. [6] Agrawal S,Cerdeira AS,Redman C,et al.Meta-analysis and systematic review to assess the role of soluble FMS-like tyrosine kinase-1 and placenta growth factor ratio in prediction of preeclampsia:the saPPPhirE study[J].Hypertension,2018,71(2):306~316. [7] 郑海娜,赵维英.检测sFlt-1、PIGF、25-羟基维生素D、D-二聚体诊断子痫前期价值[J].中国计划生育学杂志,2018,26(12):1235~1238. [8] Song S,Yang Y,Liu M,et al.MiR-125b attenuates human hepatocellular carcinoma malignancy through targeting SIRT6[J].Am Cancer Res,2018,8(6):993~1007. [9] Nie J,Jiang HC,Zhou YC,et al.MiR-125b regulates the proliferation and metastasis of triple negative breast cancer cells via the Wnt/beta-catenin pathway and EMT[J].Biosci Biotechnol Biochem,2019,83(6):1062~1071. [10] 李建华,韩玲,杨志良.miR-125b调控ART4蛋白对肝癌细胞增殖和侵袭的影响[J].中国免疫学杂志,2017,33(12):1765~1770.
2020, Vol. 26 
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