Mechanisms of Long Non-coding RNA LINC01133 Promoting the Progression of Laryngeal Squamous Cell Carcinoma Through Epithelial-Mesenchymal Transformation
CAO Huan, et al
The Second Hospital of Hebei Medical University, Hebei Shijiazhuang 050011, China
Abstract：Objective: To analyze the expression level, functions, and regulatory mechanisms of long non-coding RNA LINC01133 in laryngeal squamous cell carcinoma (LSCC).Methods: The relative expression levels of LINC01133 in 48 LSCC tissues were measured by qRT-PCR.And the associations between LINC01133 expression levels and clinic-pathological features were analyzed.After that, a series of in vitro assays were adopted to detect the effects of LINC01133 on cellular proliferation, migration, invasion ability and epithelial-mesenchymal transformation.Results: Compared to control groups, LINC01133 was highly expressed in LSCC tissues and cell lines (P<0.05).Correlation analyses showed that the expression levels of LINC01133 were associated with differentiation degree, clinical stage, and cervical lymph node metastasis (P<0.05).Besides, in vitro assays showed that LINC01133 could enhance the proliferation, migration, invasion ability and epithelial-mesenchymal transformation of LSCC cell lines.Conclusion: LINC01133 is highly expressed in LSCC tissues and cell lines, and related to the clinical stage, differentiation degree, and neck lymph node metastasis.And, LINC01133 could promote the malignant phenotype and epithelial-mesenchymal transformation of LSCC cells, which is of great significance in the occurrence and development of LSCC.
[1] 赵雷,池伟伟,曹欢,等.长链非编码RNA LINC00152在喉鳞状细胞癌中的表达及其临床意义[J].临床耳鼻咽喉头颈外科杂志,2019,33(8):721~725. [2] Weng YC,Ma J,Zhang J,et al. Long non-coding RNA LINC01133 silencing exerts antioncogenic effect in pancreatic cancer through the methylation of DKK1 promoter and the activation of Wnt signaling pathway[J]. Cancer Biol Ther,2019,20(3):368~380. [3] Zheng YF,Zhang XY,Bu YZ. LINC01133 aggravates the progression of hepatocellular carcinoma by activating the PI3K/AKT pathway[J].Cell Biochem,2019,120(3):4172~4179. [4] Liu M, Shen C, Wang C. Long Noncoding RNA LINC01133 Confers Tumor-Suppressive Functions in Ovarian Cancer by Regulating Leucine-Rich Repeat Kinase 2 as an miR-205 Sponge[J]. Am Pathol, 2019, 189(11):2323~2339. [5] Zhang C,Nie FQ,Wang Q,et al. Long non-coding RNA LINC01133 represses KLF2, P21 and E-cadherin transcription through binding with EZH2, LSD1 in non small cell lung cancer[J]. Oncotarget,2016,7(10):11696~11707. [6] Meng W,Cui W,Zhao L,et al. Aberrant methylation and down regulation of ZNF667-AS1 and ZNF667 promote the malignant progression of laryngeal squamous cell carcinoma[J].Biomed Sci,2019,26(1):13. [7] Cui W,Meng W,Zhao L,et al. TGF-β-induced long non-coding RNA MIR155HG promotes the progression and EMT of laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis[J]. Int Oncol,2019,54(6):2005~2018. [8] Zhao YQ,Liu XB,Xu H,et al. MEG3 inhibits cell proliferation, invasion and epithelial-mesenchymal transition in laryngeal squamous cell carcinoma[J]. Eur Rev Med Pharmacol Sci,2019,23(5):2062~2068. [9] Qian W, Cai X, Qian Q. Sirt1 antisense long non-coding RNA attenuates pulmonary fibrosis through sirt1-mediated epithelial-mesenchymal transition[J]. Aging (Albany NY), 2020, 12(5):4322~4336.
2020, Vol. 26 
冀公网安备13080202000677号