An Analysis of the Effects of MicroRNA-663b on Cell Activity and Apoptosis of Human Intervertebral Disc Degeneration Nucleus Pulposus Cells Induced by Low Glucose
WANG Peng, ZUO bin, TANG Jiaguo, et al
Changjiang Shipping General Hospital, Hubei Wuhan 430014, China
Abstract：Objective: To study the regulatory mechanism of miR-663b on the proliferation and apoptosis of human intervertebral disc degeneration nucleus pulposus cells induced by low glucose. Methods: The expression of miR-663b in degenerative nucleus pulposus cells of human disc herniation and glucose (5, 1 mmol/L) -treated discs was detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments. The low glucose group + miR-NC group (transfected with miR-NC), the low glucose group + miR-663b group (transfected with miR-663b mimics), and the low glucose group + anti-miR-NC group (transfected with anti-miR -NC), low glucose group + anti-miR-663b group (transfected with anti-miR-663b), divided with the plastid method, were transfected into 1 mmol/L treated disc degeneration nucleus pulposus cells. Cell Counting Kit (CCK-8) method, flow cytometry, and western blot were used to detect the absorbance (OD) value of cells, the apoptosis rate of cells, and cell cycle-dependent protein kinase inhibitor 1A (P21) , protein expression of cysteine aspartic protease (Caspase-3) in cells. Results: The expression of miR-663b in tissues of human disc herniation and 1 mmol/L glucose (low glucose group) treated disc degeneration nucleus pulposus cells were significantly reduced (P<0.05). The cells of the low glucose group were inhibited from proliferating, their apoptotic capacity was improved, and the protein expression of P21 and Caspase-3 was increased. The expression of miR-663b was significantly increased in the low-glucose group cells overexpressing miR-663b, the cell proliferation ability was also significantly increased, and the apoptotic capacity was significantly reduced. The protein expression levels of P21 and Caspase-3 were inhibited, while miR-663b's low-glucose group cells had opposite effect. Conclusion: MiR-663b can promote the proliferation of human intervertebral disc degeneration nucleus pulposus cells induced by low glucose and inhibit apoptosis.
王鹏, 左斌, 唐家国, 何精选, 何精选. miR-663b对低糖诱导的人椎间盘退变髓核细胞的细胞活性及凋亡的影响[J]. 河北医学, 2020, 26(8): 1291-1295.
WANG Peng, ZUO bin, TANG Jiaguo, et al. An Analysis of the Effects of MicroRNA-663b on Cell Activity and Apoptosis of Human Intervertebral Disc Degeneration Nucleus Pulposus Cells Induced by Low Glucose. HeBei Med, 2020, 26(8): 1291-1295.
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