Abstract：Objective: To investigate the regulation of miR-221/222 on the expression and the relation with heart failure. Methods: The peripheral blood samples from male heart failure patients and healthy volunteers were collected and separated for blood cells and plasma, respectively. The genome DNA was extracted, amplified and sequenced for analyzing the distribution of SNP genotypes in the heart failure patients and healthy controls. The plasma miRNA was extracted and the expression of miR-221/222 was detected with a TaqMan assay. The plasma levels of miR-221/222 were analyzed according to the SNP genotypes in heart failure. Results: The distribution of SNP genotypes of rs2858059、rs2858060、rs2858061 and rs34678647 were all comparable between heart failure patients and healthy controls. The genotype of rs113054794 was only C/- in all of the study subjects. The rs182069485 and rs72808106 in the 3'UTR of p27 and DDIT4 encoding genes showed no polymorphism in all of the subjects. The plasma levels of miR-221 and miR-222 were increased in heart failure patients. More importantly, we observed that the T/- genotype of rs34678647 in miR-222 was related with the increase of plasma miR-222 in heart failure patients. Conclusion: Plasma miR-221/222 levels are increased in male heart failure patients. The genotype of rs34678647 is related with the plasma miR-222 levels in heart failure patients.
[1] Verjans R, Peters T, Beaumont FJ, et al. MicroRNA-221/222 family counteracts myocardial fibrosis in pressure overload-induced heart failure [J]. Hypertension,2018,71(2):280~288. [2] Liu J, Xu Z, Li Y,, et al. Comparison between massARRAY and pyrosequencing for CYP2C19 and ABCB1 gene variants of clopidogrel efficiency genotyping[J]. Mol Membr Biol,2019,35(1):1~8. [3] Ndadza A, Cindi Z, Makambwa E, et al. Warfarin dose and CYP2C gene cluster: an african ancestral-specific variant Is a strong predictor of dose in black south african patients[J]. OMICS,2019,23(1):36~44. [4] Ni HZ, Liu Z, Sun LL,, et al. Metformin inhibits angiogenesis of endothelial progenitor cells via miR-221-mediated p27 expression and autophagy[J]. Future Med Chem,2019,11(17):2263~2272. [5] Li Z, Yu Z, Meng X, et al. Long noncoding RNA GAS5 impairs the proliferation and invasion of endometrial carcinoma induced by high glucose via targeting miR-222-3p/p27[J]. Am Transl Res,2019,11(4):2413~2421. [6] Liu J, Yu J, Jiang W, et al. Targeting of CDKN1B by miR-222-3p may contribute to the development of intervertebral disc degeneration[J]. FEBS Open Bio,2019,9(4):728~735.
2020, Vol. 26 
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