Analysis of the Levels of Serum IL-17 IL-35 in Patients with Ventilator-Associated Pneumonia Caused by Acinetobacter Baumannii and Their Connection with Prognosis
Abstract：Objective: To detect the levels of IL-17 and IL-35 in patients with ventilator-associated pneumonia (VAP) caused by Acinetobacter baumannii (AB), and to study the connection between them and the prognosis. Methods: 127 patients with VAP caused by AB in Shenzhen People's Hospital from July 2016 to October 2018 were selected as the subjects for the retrospective analysis. According to the 30-day survival of patients, they were divided into survival group (84 cases) and death group (43 cases). The general data of the two groups were collected and compared, which include:the levels of IL-17 and IL-35 in serum were detected by enzyme-linked immunosorbent assay (ELISA); T lymphocyte subsets were detected by flow cytometry; Pearson method was used to analyze the correlations between IL-17, IL-35 and T lymphocyte subsets, APACHE II score; Logistic analysis was used to analyze the factors influencing the prognosis of VAP patients caused by AB. Results: There was no significant difference in gender, age, time of tracheotomy, incision size and intraoperative hemorrhage between the two groups (P > 0.05); compared with the survival group, the APACHE II score, the levels of IL-17, IL-35 and CD8+ T lymphocyte in the dead group increased (P < 0.05); the levels of CD3+ T, CD4+ T lymphocytes and the ratio of CD4+/CD8+ decreased (P < 0.05). Pearson analysis showed that IL-17 and IL-35 were negatively correlated with CD3+ T, CD4+ T and CD4+/CD8+ (P < 0.05), and positively correlated with CD8+ T and APACHE II score (P < 0.05). The IL-17, IL-35 and APACHE II score were the risk factors for the prognosis of VAP induced by AB, and CD4+/CD8+ was the protective factor for the prognosis of VAP induced by AB. Conclusions: The increase of the levels of IL-17 and IL-35 in VAP patients is closely related to immune function factors, which is the risk factor for death. They can be used as indicators to predict the prognosis of VAP patients induced by AB.
罗钰填, 吕常艳, 谢嶷松, 刘雪燕. 鲍曼不动杆菌所致呼吸机相关性肺炎患者血清IL-17 IL-35水平及与预后关系[J]. 河北医学, 2020, 26(6): 932-935.
LUO Yutian, LV Changyan, XIE Yisong, et al. Analysis of the Levels of Serum IL-17 IL-35 in Patients with Ventilator-Associated Pneumonia Caused by Acinetobacter Baumannii and Their Connection with Prognosis. HeBei Med, 2020, 26(6): 932-935.
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2020, Vol. 26 
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