Abstract：Objective: To study the expression of miR-655-3p in head and neck squamous cell carcinoma (HNSCC) tissues and cell lines and its effects on the biological functions of HNSCC cell lines. Methods: The expression levels of miR-655-3p in HNSCC tissues and cell lines (FaDu, SCC-9, TU686, TU212) were measured by qRT-PCR, and the effects of miR-655-3p on cellular proliferation, migration and invasion were evaluated by a series of assays. The possible target gene of miR-655-3p was further screened and its mechanism was explored. Results: The expression level of miR-655-3p in HNSCC was lower than that in control group, P<0.05. And the over-expression of miR-655-3p in HNSCC cell lines significantly inhibited the proliferation, migration and invasion of cell lines. Further mechanism studies showed that ZEB1, as the target gene of miR-655-3p, was negatively regulated by miR-655-3p. Even, miR-655-3p inhibited the epithelial mesenchymal transformation of HNSCC cell lines by targeting ZEB1 expression directly. Conclusion: MiR-655-3p is down-regulated in HNSCC cancer tissues and cell lines, which could target the expression of ZEB1 directly and inhibit the progression of malignant phenotype of cell lines.
赵雷, 肖二彬, 梁景卫, 刘会清, 刘丽娜, 田从哲. miR-655-3p通过靶向抑制ZEB1对头颈鳞状细胞癌细胞增殖及侵袭能力的影响[J]. 河北医学, 2020, 26(6): 921-924.
ZHAO Lei, XIAO Erbin, LIANG Jingwei, et al. Effects of miR-655-3p on the Cellular Proliferation and Invasion of Head and Neck Squamous Cell Carcinoma Through ZEB1 Targeted Inhibition. HeBei Med, 2020, 26(6): 921-924.
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2020, Vol. 26 
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