Abstract：Objective: To study of the association between polymorphism of the MMP-9 gene(-1562C/T)and type 2 diabetic retinopathy. Methods: MMP-9 -1562C/T SNP was genotyped by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) ,analysis in 180 healthy persons as control and 400 patients with type 2 diabetes including 180 complication-free cases and 220 with diabetic retinopathy (DR). Results: Association between the MMP-9 -1562C/T SNP in promoter region and diabetic retinopathy: the C and T allele frequencies in Control, DM, DR groups are 8.3%,91.7%; 7.8%, 92.2%; 91.4%, 8.6% respectively. And the results have a significant deviation (χ2=790.09,P=0.000). The C/C, C/T , T/T genotypic frequencies of cases in the three groups are 0%,16.7%,83.3%;2.2%,11.1%,86.7%;84.1%,14.5%,1.4%.There's a significant deviation of C and T allele frequency of MMP-9 -1562C/T between the Control group、the DM group and DR group(χ2=470.16,P=0.000).There's a significant deviation of C/C,C/T,T/T genotypic frequencies between the Control group and DM group(P<0.01),or the DM group and DR group(P<0.01),while there is no significant deviation between the Control group and DM group(P>0.01) .Compared with the T allele, the C allele may increase the onset risk of diabetic retinopathy. Conclusion: The MMP-9 -1562C/T SNP may play a role in the genetic susceptibility to the invasion of diabetic retinopathy. T allele is a protective gene for diabetic retinopathy.
杨洁, 马英慧, 董微丽, 崔秀成, 石晶, 贾淑君. 基质金属蛋白酶-9基因多态性与2型糖尿病视网膜病变的相关性研究[J]. 河北医学, 2017, 23(10): 1663-1666.
YANG Jie, MA Yinghui, DONG Weili, et al. A Study of the Association between Polymorphisms of the MMP-9 Gene |and type 2 Diabetic Retinopathy. 河北医学, 2017, 23(10): 1663-1666.
[1]翟红艳,王亚娇.趋化素、肿瘤坏死因子α与2型糖尿病视网膜病变的相关性[J].眼科新进展,2015,35(7):667~669. [2] 李丰,游志鹏,谢琳.糖尿病视网膜病变危险因素及基因多态性研究进展[J].中国实用眼科杂志,2015,33(1):6~8. [3] 刘丽萍,朱吉伟,熊毅,等.上海市淞南社区糖尿病居民中糖尿病视网膜病变患病率及其影响因素调查分析[J].中华眼底病杂志,2015,31(2):126~129. [4] 郭立新,汪恕萍,李萍,等.基质金属蛋白酶9与糖尿病大血管病变的关系[J].中华内分泌代谢杂志,2002,18(6):471~472. [5] Pollanen PJ, Karhunen PJ, Mikkelsson J, et al. Coronary artery complicated lesion area is related to functional polymorphism of matrix metalloproteinase9gene: an autopsy study[J].ArteriosclerThrombVascBiol, 2001,21(9):1446~1450. [6] WHO.Report of the expert committee on diagnosis and classification of diabetics mellitus[J].Diabetics Care, 2010, 20:1183~1197. [7] 中华医学会眼科学会眼底病学组.我国糖尿病视网膜病变临床诊疗指南[J].中华眼科杂志,2014,50(11):851~864. [8] Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells[J].Nucleic Acid Res 1988,16(3):1215. [9] IDF.DiabetesAltas,6thed. Brussels: International Diabetes Federation EB.http://www.idf.org/diabetesatlas[J].2014. [10] 宁光,毕宇芳.营养与糖尿病并发症-达能营养中心第十六届学术会议论文集[C].2013:1~5. [11] Ivarsen A,Hjortdal J. Seven-year changes in corneal power and aberrations after PRK or LASIK[J].Invest Ophthalmol Vis Sci,2012,53(10):6011~6016. [12] 王宁,许迅.糖尿病视网膜病变相关基因多态性研究进展[J].中华眼科杂志,2006,42(6):563~566. [13] Nagase H, Woessner JF Jr.Matrixmetalloproteinases [J].Biol Chem,1999,274(31):21491~21494. [14] Kosano H, Okano T, Katsura Y, et al.ProMMP-9(92 kDagelatinase)in vitreous fluid of patients with proliferative diabetic retinopathy[J].Life Sci,1999,64(25):2307~2315. [15] 潘明麟,张晓梅,孙卫华,等.MMP-9-1562C/T基因多态性与2型糖尿病肾病关系的研究[J].天津医药,2010,38(8):644~647. [16] Zhang B, Ye S, Herrmann SM, etal.Functional polymorphism in the regulatory region of gelatinaseB gene in relation to severity of coronary atherosclerosis[J].Circulation,1999,99(14):1788~1794.
2017, Vol. 23 
冀公网安备13080202000677号