Abstract：Objective: To investigate the correlation between vascular endothelial growth factor (VEGF) expression and genetic polymorphism of gestational diabetes mellitus (GDM). Methods: 76 patients with GDM pregnant women who came to our hospital during the two-year period from February 2016 to 2018 and 76 normal pregnant women with normal pregnancy were selected for study. Among them, GDM pregnant women were used as observation group, and normal pregnant women were used as control group. The level of serum VEGF was determined by immunosorbent assay. The VEGF-460C/T gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. The serum VEGF level and the distribution of vegf-460c / T genotype were compared between the two groups. Results: The levels of FPG, glycated hemoglobin and VEGF in the observation group were significantly higher than those in the control group, but the HDL-C level was significantly lower than that in the control group (P<0.05). There were no significant differences in age, BMI, neonatal birth weight, TC, TG and LDL-C between the two groups (P>0.05). The distribution of VEGF-460C/T genotype CC, CT, TT and allele C and T in the two groups of pregnant women accorded with Hardy-Weinberg equilibrium. The CC genotype and C allele frequency (27.63%, 51.32%) of the observation group were significantly higher than those of the control group (10.53%, 34.87%) (P<0.05). The incidence rates of GDM in pregnant women with CC, CT and TT were 72.41 (21/29), 49.31% (36/73) and 47.50% (19/40), respectively. The incidence of GDM in CC women was significantly higher than that in CT and TT. Pregnant women (χ2=4.492, χ2=4.283, P<0.05). The results of binary logistic multivariate regression analysis showed that serum VEGF levels, HDL-C and VEGF gene polymorphisms were independent risk factors for GDM in pregnant women. Conclusion: In summary, serum VEGF level and VEGF gene polymorphism are independent risk factors for GDM in pregnant women, and C allele may be the susceptible gene of GDM.
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2020, Vol. 26 
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