两种检测血型抗体IgG亚型的方法比较

doi:10.3969/j.issn.1006-6233.2020.05.018

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摘要 目的:分别应用微柱凝胶法(MGTA)和酶联免疫技术(ELISA)检测血型抗体IgG亚型,评价两种方法在血型抗体IgG亚型检测中的应用效果以及IgG亚型与新生儿溶血病的关系。 方法:采用两种方法平行试验,分别对62例夫妇血型不合高效价(ABO系统效价≥512,Rh系统≥64)孕妇的血液标本进行血型抗体IgG亚型分析,其中ABO血型不合57例,Rh不合5例,对27例溶血三项试验确诊和可疑HDN的新生儿血样本进行IgG亚型检测,比较两种方法在检测IgG亚型方面的一致性和相关性。结果:两种方法检测的一致性较好,5例Rh血型检测结果完全一致,57例ABO血型两种方法结果一致的有44例,微柱法比ELISA法检测结果高的有6例,低的有7例,二者比较差异无显著性(P>0.05),新生儿与其母亲血清中各亚型含量呈正相关,溶血患儿血清中IgG1、IgG3明显升高,且含量越高黄疸发病越早也越严重。 结论:两种方法都可以用于检测血型抗体IgG亚型,采用微柱凝胶技术检测血型抗体IgG亚型方法相比ELISA方法操作更加简单方便,结果准确可靠,适合临床常规应用,对早期干预治疗母婴血型不合新生儿溶血病具有指导意义。

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关键词 ：微柱凝胶检测技术, 血型抗体, IgG亚型, 新生儿溶血病

Abstract：Objective: Apply the micro column gel technique assay ( MGTA) and the ELISA in detection the blood type antibody IgG subsets,evaluate the application effect to screening the antibody IgG subsets and the relationship between the antibody IgG subsets and HDN.Methods: By means of microcolum gel test and ELISA,IgG subsets were analyzed in 62 pregnant women who had the IgG anti-A(B) titer higher than 512 in 57 cases O maternal serum and the IgG anti-D titer higher than 64 in 5 cases Rh negative maternal serum.The 27 newborn cases were screened the IgG subsets and the three tests. The consistency and relativity of two methods were compared. Results:The consistency of two tests was well. The results of 5 cases Rh and 44 in 57 ABO cases were accorded.The results higher of MGTA were 6 cases and lower were 7 cases compared to ELISA, while there was no significant difference between MGTA and ELISA(P>0.05).The content of antibody IgG subsets in newborn was closely related with the mather.The IgG1 and IgG3 in postnatal haemolytic newborns were higher distinctly and the more content high the more jaundice early and serious.Conclusion: The two technology all should be used in screening the IgG antibody subsets. As for a general clinical application,the MGTA was more advantaged than ELISA because of higher accuracy and easier procedure in screening the contents of IgG antibody subsets. The method may provide a directional significance in early intervention of blood group incompatibility hemolytic disease of the newborns.

Key words： Microcolum gel technology assay(MGTA) Blood type antibody IgG subsets Hemolytic disease of the newborn(HDN)

基金资助:河北省科技计划项目,(编号:20170956)

通讯作者: 李莉华

引用本文:

马印图, 陈莉, 张怡, 董书文, 王振雷, 杨晓亚, 王少文, 李莉华. 两种检测血型抗体IgG亚型的方法比较[J]. 河北医学, 2020, 26(5): 775-778.
MA Yintu, et al. Clinical Comparison of Two Technology Assay Results in Detection of the Blood Type Antibody IgG Subsets. HeBei Med, 2020, 26(5): 775-778.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2020.05.018 或 http://www.hbyxzzs.cn/CN/Y2020/V26/I5/775