Abstract：Objective: To investigate the effects and the mechanism of DAPT blocking and NICD adenovirus activating Notch signaling pathway on proliferation, invasion and migration of prostate cancer PC-3 cells and its possible mechanism. Methods: Efficiency of DAPT blocking and AdNICD activating the Notch signaling pathway was detected by real-time PCR. EdU assay was used to evaluate the cell proliferation. Transwell assay and wound healing assay were used to detect the invasion and migration ability of PC-3 cells, respectively. p-AKT protein expression was detected by using western blot assay. Results: DAPT effectively blocked Notch signaling pathway. After adding DAPT to prostate cancer PC-3 cells, the proliferation, invasion and migration ability of cells decreased significantly, and the phosphorylation level of AKT reduced obviously. Infection of NICD adenovirus effectively activated Notch signaling pathway. After adding AdNICD to prostate cancer PC-3 cells, the proliferation, invasion and migration ability to cells increased significantly, and the phosphorylation level of AKT protein raised evidently. The difference was statistically significant. Conclusion: Notch signaling pathway can affect the proliferation, invasion and migration of PC-3 cells by regulating Akt signaling pathway.
李传贵, 王强. Notch信号通路对前列腺癌PC-3细胞增殖侵袭和迁移能力的影响[J]. 河北医学, 2020, 26(4): 669-673.
LI Chuangui, WANG Qiang. Effects of Notch Signaling Pathway on Proliferation Invasion and Migration of Prostate Cancer PC-3 cells. HeBei Med, 2020, 26(4): 669-673.
[1] Siegel RL, Miller KD, Jemal A.Cancer statistics, 2017[J]. CA Cancer Clin,2017,67(1):7~30. [2] Yang B, Liao GQ, Wen XF, et al. Nuclear magnetic resonance spectroscopy as a new approach for improvement of early diagnosis and risk stratification of prostate cancer[J].Zhejiang Univ Sci B,2017,18(11):921~933. [3] Brizzi MF, Defilippi P. Dll4/Notch1 signaling from tip/stalk endothelial cell specification to stroma-dependent lung tumor inhibition: a flavor of Dll4/Notch1 pleiotropy in tumor cell biology[J]. Transl Lung Cancer Res,2013,2(6):466~469. [4] Sanchez-Martin M, Ferrando A. The NOTCH1-MYC highway toward T-cell acute lymphoblastic leukemia[J]. Blood,2017,129(9):1124~1133. [5] Deng G, Zheng X, Jiang P, et al. Notch1 suppresses prostate cancer cell invasion via the metastasis-associated 1-KiSS-1 metastasis-suppressor pathway[J]. Oncol Lett,2017,14(4):4477~4482. [6] 王晴,杜君,杨庆.去势抵抗性前列腺癌的治疗进展[J].中国肿瘤临床,2016,43(17):766~769. [7] Nwabo Kamdje AH, Takam Kamga P, Tagne Simo R, et al. Developmental pathways associated with cancer metastasis: Notch, Wnt, and Hedgehog[J]. Cancer Biol Med,2017,14(2):109~120. [8] Sarkar S, Mirzaei R, Zemp FJ, et al. Activation of NOTCH signaling by tenascin-C promotes growth of human brain tumor-initiating cells[J]. Cancer Res,2017,77(12):3231~3243. [9] Kong P, Chen L, Yu M, et al. miR-3178 inhibits cell proliferation and metastasis by targeting Notch1 in triple-negative breast cancer[J]. Cell Death Dis,2018,9(11):1059. [10] Xiao YS, Zeng, Liang YK, et al. Major vault protein is a direct target of Notch1 signaling and contributes to chemoresistance in triple-negative breast cancer cells[J]. Cancer Lett,2019,440-441:156~167. [11] Su Q, Zhang B, Zhang L, et al. Jagged1 upregulation in prostate epithelial cells promotes formation of reactive stroma in the Pten null mouse model for prostate cancer[J]. Oncogene,2017, 36(5):618~627. [12] Cui J, Wang Y, Dong B, et al. Pharmacological inhibition of the Notch pathway enhances the efficacy of androgen deprivation therapy for prostate cancer[J]. Int Cancer,2018,143(3):645~656. [13] Taylor BS, Schultz N, Hieronymus H, et al. Integrative genomic profiling of human prostate cancer[J]. Cancer Cell,2010,18(1):11~22.
2020, Vol. 26 
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